IA-2 Autoantibody Human ELISA

Summary

Autoantibodies to pancreatic beta cell antigens are important serological markers of type 1 diabetes mellitus (type 1 DM). The antigens recognised by these antibodies include insulin, glutamic acid decarboxylase (GAD65 kDa isoform) and an islet cell antigen IA-2 or ICA-512.

Research topic

Autoimmunity, Diabetology - Autoimmunity

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum

Sample requirements

100 μl

Calibration Curve

Calibration range

0 – 4000 U/ml

Limit of detection

0.15 U/ml

Intra-assay

CV = 2.7%

Inter-assay

CV = 7.4%

References to summary

• . Sensitive non-isotopic assays for autoantibodies to IA-2 and to a combination of both IA-2 and GAD65. Clin Chim Acta. 2005 Jul 1;357 (1):74-83
• Smith BR, Furmaniak J, Burne P, Mitchell S, Bennett S, Collins A, Powell M, Brooking H, Amoroso MA, Ananieva-Jordanova R, Maclean C, Willis J, Chen S. Sensitive non-isotopic assays for autoantibodies to IA-2 and to a combination of both IA-2 and GAD65. Clin Chim Acta. 2005 Jul 1;357 (1):74-83

By research topic:

• Autoimmunity
• Diabetology - Autoimmunity

By molecule:

• IA-2 Autoantibody

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