IFN-alpha Human ELISA

Summary

IFNα (about 19 kDa, 166 amino acid residues) belongs to class I interferon. The interferons exhibit a huge number of biological effects. On purification of the natural human leukocyte interferons (IFNa), it was found that all fractions that exhibited antiviral activity also exhibited anti-growth activity.Serum IFNα levels can help to distinguish between children with non-specific abdominal pain or mesenteric adenitis, and those with acute appendicitis. Increased levels of IFNa were found in the majority of patients suffering from inflammatory arthropathies.

Research topic

Cytokines and chemokines and related molecules

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Cell culture supernatant, Plasma, Serum

Sample requirements

20 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

7.8 – 500 pg/ml

Limit of detection

3.2 pg/ml

Intra-assay

CV = 4.0%

Inter-assay

CV = 7.2%

Spiking Recovery

92%

Dilution Linearity

108%

By research topic:

• Cytokines and chemokines and related molecules

By molecule:

• IFN-alpha

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