IFN-gamma Mouse ELISA

Summary

IFNγ is a homodimeric glycoprotein containing approximately 21 to 24 kD subunits. Production of IFNg is a function of T cells and NK cells. IFNγ is produced during infection by T cells of the cytotoxic/sup­pressor phenotype (CD8) and by a subtype of helper T cells, the Th1 cells. IFNγ is a diagnostic tool for distinguishing tuberculous from other nontuberculous ascites. It is important in the design and monitoring of immunotherapy for multiple sclerosis.

Research topic

Animal studies, Cytokines and chemokines and related molecules

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Serum

Sample requirements

50 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

15.6 – 1000 pg/ml

Limit of detection

5.3 pg/ml

Intra-assay

CV = 5.0%

Inter-assay

CV = 0.1%

Spiking Recovery

76%

Dilution Linearity

107%

By research topic:

• Animal studies
• Cytokines and chemokines and related molecules

By molecule:

• IFN-gamma