IGFBP-3 Total Human ELISA

Summary

IGFBP-3 has proved to be an additional useful test in the repertoire of diagnostic tools for evaluation of growth disorders.

Features

• Quantitative determination of IGFBP-3 without sample pretreatment
• Inter-Assay variation of 6.30% and Intra-Assay variation of 4.51%
• Sensitivity of 0.1 ng/ml
• Measures growth hormone (GH)-dependent IGFBP-3 Bindingprotein
• 2 Control Sera are provided for quality control purposes according GLP
• Stable serum levels due to absence of circadian variation
• Integrates the GH secretory state over days
• A single measurement is highly informative for diagnosis of GH deficiency or GH excess
• Ideal for the diagnosis of GH-deficiency in young children
• Small sample requirement, thus ideal for pediatric patients.

Research topic

Growth hormone and factor-related products

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cerebrospinal fluid, Plasma-Heparin, Serum

Storage/Shipping

+4°C/ Wet ice

Calibration Curve

Calibration range

0.4 to 30 ng/ml

Limit of detection

0.1 ng/ml

References to this product

• Christiansen JS, Ranke MB, Moller N, Blum WF, Jorgensen JO. Circadian patterns of serum insulin-like growth factor (IGF) II and IGF binding protein 3 in growth hormone-deficient patients and age- and sex-matched normal subjects. Acta Endocrinol (Copenh). 1990 Sep;123 (3):257-62
• Cowell CT, Baxter RC. Diurnal rhythm of growth hormone-independent binding protein for insulin-like growth factors in human plasma. J Clin Endocrinol Metab. 1987 Sep;65 (3):432-40

References to summary

• Baxter RC. Characterization of the acid-labile subunit of the growth hormone-dependent insulin-like growth factor binding protein complex. J Clin Endocrinol Metab. 1988 Aug;67 (2):265-72
• Baxter RC, Holman SR. Insulin-like growth factor binding protein-3: factors affecting binary and ternary complex formation. Growth Regul. 1996 Mar;6 (1):42-7
• Bierich JR, Zeisel HJ, Gauggel E, Kietzmann K, Ranke MB, Blum WF. A specific radioimmunoassay for the growth hormone (GH)-dependent somatomedin-binding protein: its use for diagnosis of GH deficiency. J Clin Endocrinol Metab. 1990 May;70 (5):1292-8
• Martin JL, Baxter RC. Radioimmunoassay of growth hormone-dependent insulinlike growth factor binding protein in human plasma. J Clin Invest. 1986 Dec;78 (6):1504-12
• Martin JL, Baxter RC. Structure of the Mr 140,000 growth hormone-dependent insulin-like growth factor binding protein complex: determination by reconstitution and affinity-labeling. Proc Natl Acad Sci U S A. 1989 Sep;86 (18):6898-902
• Powell DR, Baxter RC, Sperry JB, Hintz RL, Lee PD. IGF binding proteins in growth-retarded children with chronic renal failure. Pediatr Res. 1989 Oct;26 (4):308-15
• Ranke MB, Blum WF. Use of insulin-like growth factor-binding protein 3 for the evaluation of growth disorders. Horm Res. 1990;33 Suppl 4:31-7
• Rosenfeld RG, Oh Y, Wilson EM. Generation and characterization of an IGFBP-7 antibody: identification of 31kD IGFBP-7 in human biological fluids and Hs578T human breast cancer conditioned media. J Clin Endocrinol Metab. 1997 Apr;82 (4):1301-3
• Schwander J, Rutanen E, Rechler M, Hintz R, Hall K, Drop S, Clemmons D, Binoux M, Baxter R, Ballard J. On the nomenclature of the IGF binding proteins. Acta Endocrinol (Copenh). 1989 Nov;121 (5):751-2

By research topic:

• Growth hormone and factor-related products

By molecule:

• IGFBP-3