IgG Rheumatoid Factor Mouse ELISA

Summary

Rheumatoid factor (RF) is an antibody (IgM or IgG) directed against the Fc region of IgG that has been used as a diagnostic marker for rheumatoid arthritis.

Research topic

Animal studies

Assay format

Direct ELISA, HRP-labelled antibody

Applications

Plasma-Citrate, Plasma-EDTA, Serum

Sample requirements

1 µl/well

Storage/Shipping

+4°C/ Wet ice

Calibration Curve

Calibration range

15.6 to 1 000 mU/ml

Limit of detection

0.78 mU/ml

References to this product

• Ishihara K, Sawa S, Ikushima H, Hirota S, Atsumi T, Kamimura D, Park SJ, Murakami M, Kitamura Y, Iwakura Y, Hirano T. The point mutation of tyrosine 759 of the IL-6 family cytokine receptor gp130 synergizes with HTLV-1 pX in promoting rheumatoid arthritis-like arthritis. Int Immunol. 2004 Mar;16 (3):455-65
• Kikukawa T, Kojima M, Abe C. A novel assay kits for autoantibodies rate on spontaneous autoimmune model mice. Jap J Inflammation. 2000;20:697-701
• Kumanogoh A, Shikina T, Watanabe C, Takegahara N, Suzuki K, Yamamoto M, Takamatsu H, Prasad DV, Mizui M, Toyofuku T, Tamura M, Watanabe D, Parnes JR, Kikutani H. Requirement for CD100-CD72 interactions in fine-tuning of B-cell antigen receptor signaling and homeostatic maintenance of the B-cell compartment. Int Immunol. 2005 Oct;17 (10):1277-82
• Oishi H, Mizuki S, Terada M, Kudo M, Araki K, Araki M, Nose M, Takahashi S. Increased expression of soluble form of vascular cell adhesion molecule-1 aggravates autoimmune arthritis in MRL-Fas(lpr) mice. Pathol Int. 2007 Nov;57 (11):734-40
• Takeda Y, Takeno M, Iwasaki M, Kobayashi H, Kirino Y, Ueda A, Nagahama K, Aoki I, Ishigatsubo Y. Chemical induction of HO-1 suppresses lupus nephritis by reducing local iNOS expression and synthesis of anti-dsDNA antibody. Clin Exp Immunol. 2004 Nov;138 (2):237-44
• Yim YK, Lee H, Hong KE, Kim YI, Lee BR, Son CG, Kim JE. Electro-acupuncture at acupoint ST36 reduces inflammation and regulates immune activity in Collagen-Induced Arthritic Mice. Evid Based Complement Alternat. 2007 Mar;4 (1):51-7

By research topic:

• Animal studies

By molecule:

• Rheumatoid Factor