IgM Rheumatoid Factor Mouse ELISA

Summary

Rheumatoid factor (RF) is an antibody (IgM or IgG) directed against the Fc region of IgG that has been used as a diagnostic marker for rheumatoid arthritis.

Assay format

Direct ELISA, HRP-labelled antibody

Applications

Plasma-Citrate, Plasma-EDTA, Serum

Sample requirements

1 µl/well

Storage/Shipping

+4°C/ Wet ice

Calibration Curve

Calibration range

15.6 to 1 000 mU/ml

Limit of detection

0.78 mU/ml

References to this product

• Kawane K, Ohtani M, Miwa K, Kizawa T, Kanbara Y, Yoshioka Y, Yoshikawa H, Nagata S. Chronic polyarthritis caused by mammalian DNA that escapes from degradation in macrophages. Nature. 2006 Oct 26;443 (7114):998-1002
• Kikukawa T, Kojima M, Abe C. A novel assay kits for autoantibodies rate on spontaneous autoimmune model mice. Jap J Inflammation. 2000;20: 697-701
• Kobayashi K, Suda T, Nan-Ya K, Sakaguchi N, Sakaguchi S, Miki I. Cytokine production profile of splenocytes derived from zymosan A-treated SKG mice developing arthritis. Inflamm Res. 2006 Aug;55 (8):335-41
• Kumanogoh A, Shikina T, Watanabe C, Takegahara N, Suzuki K, Yamamoto M, Takamatsu H, Prasad DV, Mizui M, Toyofuku T, Tamura M, Watanabe D, Parnes JR, Kikutani H. Requirement for CD100-CD72 interactions in fine-tuning of B-cell antigen receptor signaling and homeostatic maintenance of the B-cell compartment. Int Immunol. 2005 Oct;17 (10):1277-82
• Tsuji F, Yoshimi M, Katsuta O, Takai M, Ishihara K, Aono H. Point mutation of tyrosine 759 of the IL-6 family cytokine receptor, gp130, augments collagen-induced arthritis in DBA/1J mice. BMC Musculoskelet Disord. 2009;10:23
• Yamanishi S, Iizumi T, Watanabe E, Shimizu M, Kamiya S, Nagata K, Kumagai Y, Fukunaga Y, Takahashi H. Implications for induction of autoimmunity via activation of B-1 cells by Helicobacter pylori urease. Infect Immun. 2006 Jan;74 (1):248-56

By research topic:

By molecule:

• Rheumatoid Factor