Insulin Canine ELISA

Summary

Insulin (5808 Da) is a peptide hormone composed of 51 amino acid residues. It is produced in the Islets of Langerhans in the pancreas. In beta cells, insulin is synthesized from the proinsulin precursor molecule by the action of proteolytic enzymes, known as prohormone convertases, as well as the exoprotease carboxypeptidase E. Insulin is used to treat some forms of diabetes mellitus.Its principal function is to control the uptake and utilisation of glucose in peripheral tissue via the glucose transporter. This and other hypoglycaemic activities, such as the inhibition of hepatic gluconeogenesis and glycogenolysis are counteracted by the hyperglycaemic hormones including glucagon, epinephrine (adrenaline), growth hormone and cortisol.

Research topic

Animal studies, Diabetology - Insulin, C-Peptide, Proinsulin, Energy metabolism and body weight regulation

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum

Sample requirements

10 µl/well

Storage/Shipping

+4°C/ Wet ice

Calibration Curve

Calibration range

188 to 12 000 pg/ml

Limit of detection

13 pg/ml

References to this product

• Ando H, Mochiki E, Ohno T, Kogure N, Tanaka N, Tabe Y, Kimura H, Kamiyama Y, Aihara R, Nakabayashi T, Asao T, Aomori T, Fujita Y, Kuwano H. Effect of distal subtotal gastrectomy with preservation of the celiac branch of the vagus nerve to gastrointestinal function: an experimental study in conscious dogs. Ann Surg. 2008 Jun;247 (6):976-86
• Mori N, Lee P, Muranaka S, Sagara F, Takemitsu H, Nishiyama Y, Yamamoto I, Yagishita M, Arai T. Predisposition for primary hyperlipidemia in Miniature Schnauzers and Shetland sheepdogs as compared to other canine breeds. Res Vet Sci. 2010 Jun;88 (3):394-9

By research topic:

• Animal studies
• Diabetology - Insulin, C-Peptide, Proinsulin
• Energy metabolism and body weight regulation

By molecule:

• Insulin