Insulin Porcine ELISA

Summary

Insulin (5808 Da) is a peptide hormone composed of 51 amino acid residues. It is produced in the Islets of Langerhans in the pancreas. In beta cells, insulin is synthesized from the proinsulin precursor molecule by the action of proteolytic enzymes, known as prohormone convertases, as well as the exoprotease carboxypeptidase E. Insulin is used to treat some forms of diabetes mellitus.Its principal function is to control the uptake and utilisation of glucose in peripheral tissue via the glucose transporter. This and other hypoglycaemic activities, such as the inhibition of hepatic gluconeogenesis and glycogenolysis are counteracted by the hyperglycaemic hormones including glucagon, epinephrine (adrenaline), growth hormone and cortisol.

Features

• Porcine Insulin Kit is a high speed EIA. (for 3–4 hours)
• Porcine Insulin Kit can measure in samples of very small volume (10µl)
• Porcine Insulin Kit ensures simple assay procedures

Research topic

Diabetology - Insulin, C-Peptide, Proinsulin, Energy metabolism and body weight regulation

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Plasma, Serum

Sample requirements

10 µl/well

Calibration Curve

Calibration range

188 – 12000 pg/ml

Limit of detection

13 pg/ml

Spiking Recovery

95.8%

By research topic:

• Diabetology - Insulin, C-Peptide, Proinsulin
• Energy metabolism and body weight regulation

By molecule:

• Insulin