Insulin (TMB) Rat ELISA

Summary

Insulin (5808 Da) is a peptide hormone composed of 51 amino acid residues. It is produced in the Islets of Langerhans in the pancreas. In beta cells, insulin is synthesized from the proinsulin precursor molecule by the action of proteolytic enzymes, known as prohormone convertases, as well as the exoprotease carboxypeptidase E. Insulin is used to treat some forms of diabetes mellitus.Its principal function is to control the uptake and utilisation of glucose in peripheral tissue via the glucose transporter. This and other hypoglycaemic activities, such as the inhibition of hepatic gluconeogenesis and glycogenolysis are counteracted by the hyperglycaemic hormones including glucagon, epinephrine (adrenaline), growth hormone and cortisol.

Research topic

Animal studies, Diabetology - Insulin, C-Peptide, Proinsulin, Energy metabolism and body weight regulation

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture medium, Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum

Sample requirements

10 µl/well

Storage/Shipping

+4°C/ Wet ice

Calibration Curve

Calibration range

156 to 10 000 pg/ml

Limit of detection

7.8 pg/ml

References to this product

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• Imaoka M, Satoh H, Furuhama K. Age- and sex-related differences in spontaneous hemorrhage and fibrosis of the pancreatic islets in Sprague-Dawley rats. Toxicol Pathol. 2007 Apr;35 (3):388-94
• Imaoka M, Satoh H, Furuhama K. Age- and sex-related differences in spontaneous hemorrhage and fibrosis of the pancreatic islets in Sprague-Dawley rats. Toxicol Pathol. 2007;35 (3):388-94
• Ishii N, Wei M, Kakehashi A, Doi K, Yamano S, Inaba M, Wanibuchi H. Enhanced Urinary Bladder, Liver and Colon Carcinogenesis in Zucker Diabetic Fatty Rats in a Multiorgan Carcinogenesis Bioassay: Evidence for Mechanisms Involving Activation of PI3K Signaling and Impairment of P53 on Urinary Bladder Carcinogenesis. Journal of Toxicologic Patholo. Vol. 24 (2011) , No. 1 pp.25
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• Minami A, Ishimura N, Sakamoto S, Takishita E, Mawatari K, Okada K, Nakaya Y. Effect of eicosapentaenoic acid ethyl ester v. oleic acid-rich safflower oil on insulin resistance in type 2 diabetic model rats with hypertriacylglycerolaemia. Br J Nutr. 2002 Feb;87 (2):157-62
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By research topic:

• Animal studies
• Diabetology - Insulin, C-Peptide, Proinsulin
• Energy metabolism and body weight regulation

By molecule:

• Insulin