Insulin (U-E type) Rat ELISA

Summary

Insulin (5808 Da) is a peptide hormone composed of 51 amino acid residues. It is produced in the Islets of Langerhans in the pancreas. In beta cells, insulin is synthesized from the proinsulin precursor molecule by the action of proteolytic enzymes, known as prohormone convertases, as well as the exoprotease carboxypeptidase E. Insulin is used to treat some forms of diabetes mellitus.Its principal function is to control the uptake and utilisation of glucose in peripheral tissue via the glucose transporter. This and other hypoglycaemic activities, such as the inhibition of hepatic gluconeogenesis and glycogenolysis are counteracted by the hyperglycaemic hormones including glucagon, epinephrine (adrenaline), growth hormone and cortisol.

Features

• Rat Insulin ELISA (U-E type) Kit is a highly sensitive ELISA (39–2500pg/ml).
• Rat Insulin ELISA (U-E type) Kit can measure insulin in samples* of very small volume (10μl), and rapidly (3 hours)
• Rat Insulin ELISA (U-E type) Kit ensures simple assay procedures with high precision and excellent reproducibility.

• We recommend the use of heparin for plasma samples.

Research topic

Diabetology - Insulin, C-Peptide, Proinsulin, Energy metabolism and body weight regulation

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture medium, Plasma, Serum

Sample requirements

10 µl/well

Storage/Shipping

At 2–8°C. Avoid light. Do not freeze.

Calibration Curve

Calibration range

39 – 2 500 pg/ml

Limit of detection

3.9 pg/ml

Spiking Recovery

97.5%

By research topic:

• Diabetology - Insulin, C-Peptide, Proinsulin
• Energy metabolism and body weight regulation

By molecule:

• Insulin