Insulin (U-type) Mouse ELISA

Summary

Insulin (5808 Da) is a peptide hormone composed of 51 amino acid residues. It is produced in the Islets of Langerhans in the pancreas. In beta cells, insulin is synthesized from the proinsulin precursor molecule by the action of proteolytic enzymes, known as prohormone convertases, as well as the exoprotease carboxypeptidase E. Insulin is used to treat some forms of diabetes mellitus.Its principal function is to control the uptake and utilisation of glucose in peripheral tissue via the glucose transporter. This and other hypoglycaemic activities, such as the inhibition of hepatic gluconeogenesis and glycogenolysis are counteracted by the hyperglycaemic hormones including glucagon, epinephrine (adrenaline), growth hormone and cortisol.

Features

• Mouse Insulin ELISA (U-type) Kit is a highly sensitive ELISA (39～2500pg/ml).
• Mouse Insulin ELISA (U-type) Kit can measure insulin in samples of very small volume(5µl).
• Mouse Insulin ELISA (U-type) Kit ensures simple assay procedures.

Research topic

Diabetology - Insulin, C-Peptide, Proinsulin, Energy metabolism and body weight regulation

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture medium, Plasma, Serum

Sample requirements

5 µl/well

Calibration Curve

Calibration range

39 – 2 500 pg/ml

Limit of detection

3.9 pg/ml

Spiking Recovery

CV = 100.9%

References to this product

• Cho EJ, Hwang HJ, Kim SW, Oh JY, Baek YM, Choi JW, Bae SH, Yun JW. Hypoglycemic effects of exopolysaccharides produced by mycelial cultures of two different mushrooms Tremella fuciformis and Phellinus baumii in ob/ob mice. Appl Microbiol Biotechnol. 2007 Jul;75 (6):1257-65
• Gomi H, Mizutani S, Kasai K, Itohara S, Izumi T. Granuphilin molecularly docks insulin granules to the fusion machinery. J Cell Biol. 2005 Oct 10;171 (1):99-109
• Hong H, Jai Maeng W. Effects of malted barley extract and banaba extract on blood glucose levels in genetically diabetic mice. J Med Food. 2004 Winter;7 (4):487-90
• Inoue M, Sakuraba Y, Motegi H, Kubota N, Toki H, Matsui J, Toyoda Y, Miwa I, Terauchi Y, Kadowaki T, Shigeyama Y, Kasuga M, Adachi T, Fujimoto N, Matsumoto R, Tsuchihashi K, Kagami T, Inoue A, Kaneda H, Ishijima J, Masuya H, Suzuki T, Wakana S, Gondo Y, Minowa O, Shiroishi T, Noda T. A series of maturity onset diabetes of the young, type 2 (MODY2) mouse models generated by a large-scale ENU mutagenesis program. Hum Mol Genet. 2004 Jun 1;13 (11):1147-57
• Jin W, Takagi T, Kanesashi SN, Kurahashi T, Nomura T, Harada J, Ishii S. Schnurri-2 controls BMP-dependent adipogenesis via interaction with Smad proteins. Dev Cell. 2006 Apr;10 (4):461-71
• Kasai K, Ohara-Imaizumi M, Takahashi N, Mizutani S, Zhao S, Kikuta T, Kasai H, Nagamatsu S, Gomi H, Izumi T. Rab27a mediates the tight docking of insulin granules onto the plasma membrane during glucose stimulation. J Clin Invest. 2005 Feb;115 (2):388-96
• Manabe Y, Utsunomiya H, Gotoh K, Kurosu T, Fushiki T. Relationship between the preference for sake (Japanese rice wine) and the movements of metabolic parameters coinciding with sake intake. Biosci Biotechnol Biochem. 2004 Apr;68 (4):796-802
• Miyake K, Ogawa W, Matsumoto M, Nakamura T, Sakaue H, Kasuga M. Hyperinsulinemia, glucose intolerance, and dyslipidemia induced by acute inhibition of phosphoinositide 3-kinase signaling in the liver. J Clin Invest. 2002 Nov;110 (10):1483-91
• Miyawaki K, Inoue H, Keshavarz P, Mizuta K, Sato A, Sakamoto Y, Moritani M, Kunika K, Tanahashi T, Itakura M. Transgenic expression of a mutated cyclin-dependent kinase 4 (CDK4/R24C) in pancreatic beta-cells prevents progression of diabetes in db/db mice. Diabetes Res Clin Pract. 2008 Oct;82 (1):33-41
• Mizutani S, Gomi H, Hirayama I, Izumi T. Chromosome 2 locus Nidd5 has a potent effect on adiposity in the TSOD mouse. Mamm Genome. 2006 May;17 (5):375-84
• Sung YY, Lee YS, Jung WH, Kim HY, Cheon HG, Yang SD, Rhee SD. Glucose intolerance in young TallyHo mice is induced by leptin-mediated inhibition of insulin secretion. Biochem Biophys Res Commun. 2005 Dec 30;338 (4):1779-87
• Tanaka N, Moriya K, Kiyosawa K, Koike K, Gonzalez FJ, Aoyama T. PPARalpha activation is essential for HCV core protein-induced hepatic steatosis and hepatocellular carcinoma in mice. J Clin Invest. 2008 Feb;118 (2):683-94
• Tsukiyama K, Yamada Y, Miyawaki K, Hamasaki A, Nagashima K, Hosokawa M, Fujimoto S, Takahashi A, Toyoda K, Toyokuni S, Oiso Y, Seino Y. Gastric inhibitory polypeptide is the major insulinotropic factor in K(ATP) null mice. Eur J Endocrinol. 2004 Sep;151 (3):407-12

By research topic:

• Diabetology - Insulin, C-Peptide, Proinsulin
• Energy metabolism and body weight regulation

By molecule:

• Insulin