Interleukin-1 alpha Mouse ELISA

Summary

IL-1α is predominantly a cell-associated, non-secreted proinflammatory cytokine. It is produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. High levels of IL-1α are associated with sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, and atherosclerosis.

Research topic

Animal studies, Cytokines and chemokines and related molecules

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Serum

Sample requirements

50 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

7.8 – 500 pg/ml

Limit of detection

3.9 pg/ml

Intra-assay

CV = 5.0%

Inter-assay

CV = 10.0%

Spiking Recovery

75%

Dilution Linearity

101%

By research topic:

• Animal studies
• Cytokines and chemokines and related molecules

By molecule:

• Interleukin-1 alpha