Interleukin-1 alpha Rat ELISA

Summary

IL-1α is predominantly a cell-associated, non-secreted proinflammatory cytokine. It is produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. High levels of IL-1α are associated with sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, and atherosclerosis.

Research topic

Animal studies, Cytokines and chemokines and related molecules

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Serum

Sample requirements

50 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

15.6 – 1000 pg/ml

Limit of detection

4.0 pg/ml

Intra-assay

CV = 5.0%

Inter-assay

CV = 10.0%

Dilution Linearity

112.4 %

By research topic:

• Animal studies
• Cytokines and chemokines and related molecules

By molecule:

• Interleukin-1 alpha

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