Interleukin-10 Human ELISA

Research topic

Cytokines and Chemokines and related molecules

Storage/Shipping

+4°C/ Wet ice

Summary

IL-10 is an immunoregulatory protein produced by a number of cell lineages, including TH2 cells, B-cells and activated monocytes. Il-10 is an important factor in understanding autoimmunity and as a target molecule in the development of anti-rejection therapies.

Assay format

Sandwich ELISA, Biotin-labelled antibody

Sample requirements

50 µl/well

Applications

Cell culture medium, Plasma-Citrate, Plasma-Heparin, Serum, Urine

Calibration Curve

Calibration range

7.8 to 500 pg/ml

Limit of detection

2 pg/ml

Spiking Recovery

2 pg/ml

Cross-Reactivity

No cross reactivity was observed with the following recombinant human proteins: IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-12, IL-13, TNF α, TARC.

References to summary

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• Whitcup SM et al.: Association of interleukin 10 in the vitreous and cerebrospinal fluid and primary central nervous system lymphoma.Arch. Ophthalmol. 115, 1157–1160 (1997).
• Zhang Y et al.: Secretory leukocyte protease inhibitor suppresses the production of monocyte prostaglandin H synthase-2, prostaglandin E2, and matrix metalloprotei­nases.J. CLin. Invest. 99, 894–900 (1997).