Interleukin-10 Mouse ELISA

Summary

Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. IL-10 blocks cytokine synthesis and several accessory cell functions of macrophages, T-cells and NK cells. Murine IL-10 exhibits strong DNA and aminoacid sequence homology to human IL-10 and an to an open reading frame in the Epstein- Barr virus genome, BCRF1, which shares many of the cellular cytokine's bi­ological activities and may therefore play a role in the hostvirus interaction.

Research topic

Animal studies, Cytokines and chemokines and related molecules

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Plasma, Serum

Sample requirements

50 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

15.6 – 1000 pg/ml

Limit of detection

5 pg/ml

Intra-assay

CV = 4.4%

Inter-assay

CV = 5.1%

Spiking Recovery

133%

Dilution Linearity

108%

By research topic:

• Animal studies
• Cytokines and chemokines and related molecules

By molecule:

• Interleukin-10