Interleukin-12 Human ELISA
| Other names: | IL-12, Interleukin-12 subunit p35, p40, p70, Cytotoxic lymphocyte maturation factor , CLMF, NKSF, natural killer cell stimulatory factor | ||
|---|---|---|---|
| Cat. No.: | ROIL12001R | Regulatory status: RUO | |
| Size: | 96 wells (1 kit) | | | |
| Files: | Datasheet PDF (RUO) MSDS (RUO) | ||
| Legend: |  new product discount |
 temporarily out of stock order in advance |
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Research topic
Cytokines and Chemokines and related molecules
Storage/Shipping
+4°C/ Wet ice
Summary
IL-12 (70 kDa) is an important pro-inflammatory cytokine produced by dentritic cells, macrophages and B lymphocytes and it has been shown to have multiple effects on T cells and natural killer cells. IL-12 displays significant anti-tumor activity. In vivo, IL-12 appears to play a major role in auto-immune disease, ián the resistance to bacterial and parasitic infections, and in antiviral responses including HIV.
Assay format
Sandwich ELISA, Biotin-labelled antibody
Sample requirements
50 µl/well
Applications
Cell culture medium, Plasma-Citrate, Plasma-Heparin, Serum, Urine
Calibration Curve
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Calibration range
7.8 to 500 pg/ml
Limit of detection
2 pg/ml
Spiking Recovery
2 pg/ml
Cross-Reactivity
No cross reactivity was observed with the following recombinant human proteins: IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-13, TNF α, TARC.
References to summary
- Tejde A et al.: Immune complex-stimulated production of interleukin-12 in peripheral blood mononuclear cells is regulated by the complement system.Clin. Exp. Immunol. 2004;137: 521.
- Pekkari K et al.: Truncated thioredoxin (Trx80) induces production of interleukin- 12 and enhances CD14 expression in human monocytes.Blood 2001;97: 3184.
- Wong CK, Ho CY, Li EK, Lam CW: Elevation of proinflammatory cytokine (IL- 18, IL-17, IL-12) and Th2 cytokine (IL-4) concentrations in patients with systemic lupus erythematosus.Lupus. 2000;9:589–93.
- Cordero OJ, Salgado FJ, Mera-Varela A, Nogueira M: Serum interleukin-12, interleukin-15, soluble CD26, and adenosine deaminase in patients with rheumatoid arthritis.Rheumatol Int. 2001;21:69–74.
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