Interleukin-12 Human ELISA

Research topic

Cytokines and Chemokines and related molecules

Storage/Shipping

+4°C/ Wet ice

Summary

IL-12 (70 kDa) is an important pro-inflammatory cytokine produced by dentritic cells, macrophages and B lymphocytes and it has been shown to have multiple effects on T cells and natural killer cells. IL-12 displays significant anti-tumor activity. In vivo, IL-12 appears to play a major role in auto-immune disease, ián the resistance to bacterial and parasitic infections, and in antiviral responses including HIV.

Assay format

Sandwich ELISA, Biotin-labelled antibody

Sample requirements

50 µl/well

Applications

Cell culture medium, Plasma-Citrate, Plasma-Heparin, Serum, Urine

Calibration Curve

Calibration range

7.8 to 500 pg/ml

Limit of detection

2 pg/ml

Spiking Recovery

2 pg/ml

Cross-Reactivity

No cross reactivity was observed with the following recombinant human proteins: IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-13, TNF α, TARC.

References to summary

• Tejde A et al.: Immune complex-stimulated production of interleukin-12 in peripheral blood mononuclear cells is regulated by the complement system.Clin. Exp. Immunol. 2004;137: 521.
• Pekkari K et al.: Truncated thioredoxin (Trx80) induces production of interleukin- 12 and enhances CD14 expression in human monocytes.Blood 2001;97: 3184.
• Wong CK, Ho CY, Li EK, Lam CW: Elevation of proinflammatory cytokine (IL- 18, IL-17, IL-12) and Th2 cytokine (IL-4) concentrations in patients with systemic lupus erythematosus­.Lupus. 2000;9:589–93.
• Cordero OJ, Salgado FJ, Mera-Varela A, Nogueira M: Serum interleukin-12, interleukin-15, soluble CD26, and adenosine deaminase in patients with rheumatoid arthritis.Rhe­umatol Int. 2001;21:69–74.