Interleukin-12/Interleukin-23 p40 Human ELISA

Summary

IL-23 and IL-12 are heterodimeric cytokines which share the p40 subunit. Unlike IL-12, IL-23 has no effect on naive T cells but selectively activates memory T cells to secrete IFN-γ and IL-17. IL-23 is more potent than IL-12 in inducing long-lasting T-cell responses to viral antigens and can induce tumor-directed cytotoxic T-cell responses.

Research topic

Cytokines and chemokines and related molecules

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Plasma, Serum

Sample requirements

50 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

31.3 – 2000 pg/ml

Limit of detection

< 10 pg/ml

Intra-assay

CV = 4.7%

Inter-assay

CV = 6.4%

Spiking Recovery

71%

Dilution Linearity

109.8%

By research topic:

• Cytokines and chemokines and related molecules

By molecule:

• Interleukin-12/Interleukin-23 p40