Interleukin-12 (p70) Monkey ELISA

Summary

Interleukin-12 is a pleiotropic cytokine, which is produced primarily by stimulated macrophages. IL-12 has been shown to be a proinflammatory cytokine produced by phagocytic cells, B cells, and other antigen-presenting cells that modulate adaptive immune responses by favoring the generation of T-helper type 1 cells. IL-12 exerts a variety of biological effects on T and natural killer cells. IL-12 induced IFN-gamma acts in a positive feedback loop that represents an important amplifying mechanism in the inflammatory response to infections. Its role in directing development of a Th1 type immune response from naive T cells demonstrates its critical role in regulation of the immune response and strongly suggests its potential usefulness in cancer therapy.

Research topic

Animal studies, Cytokines and chemokines and related molecules

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Baboon serum, Cell culture supernatant

Sample requirements

50 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

3.9 – 250 U/ml

Limit of detection

1.6 U/ml

Intra-assay

CV = 5.0%

Inter-assay

CV = 5.0%

Spiking Recovery

105%

Dilution Linearity

103%

By research topic:

• Animal studies
• Cytokines and chemokines and related molecules

By molecule:

• Interleukin-12