Interleukin-13 Human ELISA

Summary

Interleukin-13 (IL-13) is a Th2-type cytokine, secreted from CD4(+) T cells, mast cells, basophils and eosinophils. It is an immunoregulatory cytokine. It has become evident that IL-13 is a key mediator in the pathogenesis of allergic inflammation. The important role of IL-13 in the pathogenesis of bronchial asthma as well as other allergic diseases has been recognized. Additionally, IL-13 plays a major role in various other inflammatory diseases including cancer.

Research topic

Cytokines and chemokines and related molecules

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Amniotic fluid, Cell culture supernatant, Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum

Sample requirements

50 µl/well

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

1.6 – 100 pg/ml

Limit of detection

0.73 pg/ml

Inter-assay

CV = 6.0%

Spiking Recovery

CV = 4.6%

By research topic:

• Cytokines and chemokines and related molecules

By molecule:

• Interleukin-13