Interleukin-13 Human ELISA

Research topic

Cytokines and Chemokines and related molecules

Storage/Shipping

+4°C/ Wet ice

Summary

IL-13 (12 kDa, 132 amino acids) is a nonglycosylated immunoregulatory protein. IL-13 may play an important regulatory role in IgE-mediated atopic diseases. The measurement of IL-13 in body fluids may thus provide further information about the pathophysiology of atopic diseases.

Assay format

Sandwich ELISA, Biotin-labelled antibody

Sample requirements

50 µl/well

Applications

Cell culture medium, Plasma-Citrate, Plasma-Heparin, Serum, Urine

Calibration Curve

Calibration range

7.8 to 125 pg/ml

Limit of detection

2 pg/ml

Intra-assay

<6.2%

Inter-assay

<7.4%

Spiking Recovery

2 pg/ml

Cross-Reactivity

No cross reactivity was observed with the following recombinant human proteins: IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12, IL-15, TNF α, TARC.

References to summary

• Hauber HP, Gholami D, Koppermann G, Heuer HE, Meyer A, Pforte A: Increased expression of Interleukin-13 but not Interleukin-4 in cystic fibrosis patients.J Cyst Fibros. 2003 Dec;2(4):189–94.
• Hasegawa M, Sato S, Nagaoka T, Fujimoto M, Takehara K: Serum levels of tumor necrosis factor and interleukin-13 are elevated in patients with localized scleroderma.Der­matology. 2003;207(2):141–7.
• Hauber HP, Gholami D, Meyer A, Pforte A: Increased interleukin-13 expression in patients with sarcoidosis.Tho­rax. 2003 Jun;58(6):519–24.
• Tain YL, Chen TY, Yang KD: Implications of serum TNF-beta and IL-13 in the treatment response of childhood nephrotic syndrome.Cytokine. 2003 Feb 7;21(3):155–9.
• Nasu K, Fukuda J, Sun B, Nishida M, Miyakawa I: Interleukin-13 and tumor necrosis factor-beta differentially regulate the production of cytokines by cultured human endometrial stromal cells.Fertil Steril. 2003 Mar;79 Suppl 1:821–7.