Interleukin-17AF Human ELISA

Summary

IL-17A and IL-17F are members of the IL-17 family and signature Th17 cytokines. Of the six IL-17 family members, IL-17F and IL-17A share the strongest homology (50% amino acid identity) and the two genes are located in the same chromosomal region. Although both IL-17A and IL- 17F were originally found to be produced as disulfide-linked homodimers, recent studies have confirmed the production of IL-17A/IL-17F heterodimers in in vitro-differentiated and polarized Th17 cells. Activated human CD4+ T cells in culture were found to secrete IL-17F homodimer at 10-fold higher levels than IL-17A homodimer, suggesting that the majority of the IL-17A protein expressed exists in the form of the IL-17AF heterodimer. Studies of Th17 polarized mouse splenocytes also indicate the majority of IL-17 made is the IL-17A/F heterodimer. IL-17F and IL-17A have been observed in tissue samples from various autoimmune diseases, such as rheumatoid arthritis, multiple sclerosis, psoriasis, inflammatory bowel disease, and asthma.

Research topic

Cytokines and chemokines and related molecules

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Plasma-Citrate, Plasma-Heparin, Serum

Sample requirements

50 ul

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

31.3 – 2 000 pg/ml

Limit of detection

8.8 pg/ml

By research topic:

• Cytokines and chemokines and related molecules

By molecule:

• Interleukin-17AF