Interleukin-2 Receptor (Soluble) Human ELISA

Research topic

Cytokines and Chemokines and related molecules

Storage/Shipping

Refrigerated at 2 – 8 °C.

Summary

The receptor of the cytokine IL-2 plays a crucial role in the regulation of the immune response. Binding of IL-2 to its receptor (IL-2R) on the surface of T-lymphocytes triggers a series of intracellular signaling events that result in the activation and proliferation of resting T cells and in the generation of helper, suppressor and cytotoxic T cells, which mediate immune reactions. sIL-2R is a marker for T cell activation. It can be an indicator of a wide spectrum of disorders involving immune activation. It can be found at significantly elevated levels in a broad range of disorders such as tumor diseases, autoimmune diseases (e.g. sarcoidosis), organ rejection and different infections.

Assay format

Sandwich ELISA, HRP-labelled antibody

Sample requirements

25µl/well

Applications

Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum

Calibration Curve

Calibration range

225 to 7 200 U/ml

Limit of detection

48 U/ml

Intra-assay

CV = 6.9 %

Inter-assay

CV = 7.5 %

Spiking Recovery

96 %

References to summary

• Rubin LA: The soluble interleukin – 2 receptor: biology function, and clinical application.Ann Intern Med 1990; 113:619–27.
• Taniguchi T, Minami Y: The IL-2/IL-2 receptor system: a current overview.Cell 1993;73:5–8.
• Fernandez-Botran R: Soluble cytokine receptors: their role in immunoregulati­on.FASEB 1991 Vol 5: 2567–2574
• Pizzolo G: The soluble interleukin – 2 receptor as a new biological marker in diseases.Immunol Clin 1988; VII:1.
• Gause A, Roschansky V, Tschiersch A, Smith K, Hasenclever D, Schmits R, Diehl V, Pfreundschuh M: Low serum interleukin-2 receptor levels correlate with a good prognosis in patients with Hodgkin´s lym­phoma.Annals of Oncology,1991, 2 (2): 43–47.
• Ziegenhagen MW, Benner UK, Zissel G, Zabel P, Schlaak M, Müller-Quernheim J: Sarcoidosis: TNF-α release from alveolar macrophages and serum levels of sIL-2R are prognostic markers.Am J Respir Crit Care Med, 1997, 156: 1586–1592.