Interleukin-4 Rat ELISA

Summary

IL-4 (15 to 19 kDa) is produced by a variety of T cell thymocyte populations, as well as some mast cell precursors that are non-T or B cell types. Il-4 plays an important role in the activation of resting B-cells and in the subsequent proliferation and differentiation of B-cells. IL-4 is essential for IgE synthesis in vitro and its involvement with allergic diseases has been investigated. Il-4 has anti-inflammatory effects.

Research topic

Animal studies, Cytokines and chemokines and related molecules

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Serum

Sample requirements

50 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

1.6 – 100 pg/ml

Limit of detection

0.2 pg/ml

Intra-assay

CV = 5.0%

Inter-assay

CV = 10.0%

Dilution Linearity

94%

By research topic:

• Animal studies
• Cytokines and chemokines and related molecules

By molecule:

• Interleukin-4

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