Interleukin-6 Mouse ELISA

Summary

Interleukin-6 is a multi-functional cytokine that regulates immune responses, acute phase reactions and hematopoiesis, and it may play a central role in host defense mechanisms. IL-6 is usually not produced constitutively by normal cells, but its expression is readily induced by a variety of cytokines, lipopolysaccharide or viral infections.

Research topic

Animal studies, Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Plasma-Citrate, Plasma-EDTA, Serum

Sample requirements

50 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

31.3 – 2000 pg/ml

Limit of detection

6.5 pg/ml

Intra-assay

CV = 5.0%

Inter-assay

CV = 8.9%

References to summary

• Cayphas S, Van Damme J, Vink A, Simpson RJ, Billiau A, Van Snick J. Identification of an interleukin HP1-like plasmacytoma growth factor produced by L cells in response to viral infection. J Immunol. 1987 Nov 1;139 (9):2965-9
• Hirano T, Kishimoto T. Interleukin-6.Handbook of experimental pharmacology, Peptide Growth Factors and Their Receptors. Springer-Verlag. 1990;:633-665
• Kishimoto T, Pyun KH, Nakajima K, Shimizu K, Kashiwamura S, Yasukawa K, Nakano N, Taga T, Hirano T. Purification to homogeneity and characterization of human B-cell differentiation factor (BCDF or BSFp-2). Proc Natl Acad Sci U S A. 1985 Aug;82 (16):5490-4
• Kishimoto T, Taga T, Akira S, Hirano T. Biological and clinical aspects of interleukin 6. Immunol Today. 1990 Dec;11 (12):443-9
• Nordan RP, Potter M. A macrophage-derived factor required by plasmacytomas for survival and proliferation in vitro. Science. 1986 Aug 1;233 (4763):566-9
• Sehgal PB, Greininger G, Tosato G. Regulation of the acute phase and immune responses: interleukin-6. Ann N Y Acad Sci. 1989;557:1-583
• Sehgal PB, May LT, Helfgott DC, Santhanam U, Tatter SB, Ray A. Regulation of expression of interleukin-6. Molecular and clinical studies. Ann N Y Acad Sci. 1989;557:353-61; discussion 361-2

By research topic:

• Animal studies
• Cytokines and chemokines and related molecules
• Energy metabolism and body weight regulation

By molecule:

• Interleukin-6

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