Interleukin-8 Human ELISA

Research topic

Cytokines and Chemokines and related molecules

Summary

The chemokines can be divided into two subfamilies depending on whether the first two cysteines are adjacent (C-C chemokines) or not (C-X-C chemokines). IL-8 is a member of the second subfamily. IL-8 is a non-glycosylated protein of 8 kDa (72 amino acids) and it is produced by the processing of a precursor protein of 99 amino acids. Monocytes, macrophages, neutrophils, lymphocytes, dermal fibroblasts, keratinocytes, vascular endothelial cells, melanocytes, hepatocytes and various tumor cell lines produce IL-8. IL-8 is present in the synovial fluids of patients with rheumatoid arthritis and has been purified from plaque psoriasis scales. It is associated also with sepsis, asthma and glomerulonephritis. Elevated levels of IL-8 have been discovered after sublethal endotoxaemia, septic shock, microbial infection of the amniotic cavity, Jarish-Herxheimer reaction of relapsing fever, infectious diseases of the central nervous system, acute pancreatitis, ulcerative colitis, empyaema, haemolitic uraemic syndrome, meningococcal disease, gastric infection, pertussis, and peritonitis. IL-8 will be clinically important in understanding disease states that are characterized by neutrophil infiltration.

Assay format

Sandwich ELISA, Biotin-labelled antibody

Sample requirements

50 μl/well

Applications

Cell culture medium, Plasma-Citrate, Plasma-Heparin, Serum, Urine

Calibration Curve

Calibration range

3.9 to 25 pg/ml

Limit of detection

2 pg/ml

Limit of quantification

2 pg/ml

Intra-assay

< 5.2%

Inter-assay

< 6.4%

Cross-Reactivity

No cross reactivity was observed with the following recombinant human proteins: IL-1α, IL-1β, Il-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12, IL-13, TNF α, TARC.

References to summary

• Baggiolini M et al.: Neutrophil-activating peptide-1/Interleukin-8, a novel cytokine that activates neutrophils. J. Clin. Invest. 84:1045–1049.
• Croce MA et al.: Partial liquid ventilation decreases the inflammatory response in the alveolar environment of trauma patients. J. Trauma 45: 273–282 (1998).
• Hack C et al.: Interleukin-8 in sepsis: relation to shock and inflammatory mediators. Infect. Immun. 60:2835–2842 (1992).
• Matsuhuma K et al.: Purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line. J. Exp. Med. 169:1485–1490 (1989).
• Tilg H et al.: Interleukin-8 serum concentrations after liver transplantation. Transplant. 53:800–803 (1992).