Interleukin-8 Human ELISA

Research topic

Cytokines and chemokines and related molecules

Summary

IL-8 (8 kDa, 72 amino acids) is a non-glycosylated protein and it is produced by the processing of a precursor protein of 99 amino acids. IL-8 is present in the synovial fluids of patients with rheumatoid arthritis and has been purified from plaque psoriasis scales. It is associated with sepsis, asthma and glomerulonephritis.

Assay format

Sandwich ELISA, Biotin-labelled antibody

Sample requirements

50 μl/well

Applications

Cell culture medium, Plasma-Citrate, Plasma-Heparin, Serum, Urine

Calibration Curve

Calibration range

3.9 to 250 pg/ml

Limit of detection

7 pg/ml

Limit of quantification

7 pg/ml

Intra-assay

< 5.2%

Inter-assay

< 6.4%

Cross-Reactivity

No cross reactivity was observed with the following recombinant human proteins: IL-1α, IL-1β, Il-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12, IL-13, TNF α, TARC.

References to summary

• Baggiolini M et al.: Neutrophil-activating peptide-1/Interleukin-8, a novel cytokine that activates neutrophils. J. Clin. Invest. 84:1045–1049.
• Croce MA et al.: Partial liquid ventilation decreases the inflammatory response in the alveolar environment of trauma patients. J. Trauma 45: 273–282 (1998).
• Hack C et al.: Interleukin-8 in sepsis: relation to shock and inflammatory mediators. Infect. Immun. 60:2835–2842 (1992).
• Matsuhuma K et al.: Purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line. J. Exp. Med. 169:1485–1490 (1989).
• Tilg H et al.: Interleukin-8 serum concentrations after liver transplantation. Transplant. 53:800–803 (1992).