Interleukin-8/NAP-1Human ELISA

Summary

IL-8 (8 kDa, 72 amino acids) is a non-glycosylated protein and it is produced by the processing of a precursor protein of 99 amino acids. IL-8 is present in the synovial fluids of patients with rheumatoid arthritis and has been purified from plaque psoriasis scales. It is associated with sepsis, asthma and glomerulonephritis.

Research topic

Cytokines and chemokines and related molecules

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Cell culture supernatant, Plasma-EDTA, Plasma-Heparin, Serum

Sample requirements

50 µl/well

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

15.6 – 1 000 pg/ml

Limit of detection

2.0 pg/ml

Intra-assay

CV=6.3%

Inter-assay

Cv=8.7%

By research topic:

• Cytokines and chemokines and related molecules

By molecule:

• Interleukin-8

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