KL-6 Human ELISA

Summary

KL-6, a mucinous high-molecular weight glycoprotein, expressed on type II pneumonocytes, is elevated in the serum of patients with active interstitial pneumonia.

Research topic

Immune Response, Infection and Inflammation

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Serum

Sample requirements

10 μL

Storage/Shipping

2– 8℃

Calibration Curve

Calibration range

201 – 4 020 U/mL

Limit of detection

1 U/ml (with 2SD method)

References to summary

• Akiyama M, Yamakido M, Hiwada K, Fujioka S, Hamada H, Kohno N. Circulating antigen KL-6 and lactate dehydrogenase for monitoring irradiated patients with lung cancer. Chest. 1992 Jul;102 (1):117-22
• Akiyama M, Yamakido M, Vossler M, Oyama T, Tanabe M, Kyoizumi S, Kohno N. Detection of a circulating tumor-associated antigen with a murine monoclonal antibody, LISA 101, selected by reversed indirect enzyme-linked immunosorbent assay. Cancer Res. 1989 Jun 15;49 (12):3412-9
• Hiwada K, Akiyama M, Kohno N, Hamada H. Monitoring of serum KL-6 antigen in a patient with radiation pneumonia. Chest. 1992 Mar;101 (3):858-60
• Hiwada K, Fujioka S, Hamada H, Yokoyama A, Inoue Y, Akiyama M, Yamakido M, Oyama T, Awaya Y, Kohno N. KL-6, a mucin-like glycoprotein, in bronchoalveolar lavage fluid from patients with interstitial lung disease. Am Rev Respir Dis. 1993 Sep;148 (3):637-42
• Ichinose M, Goda M, Ichikawa T, Furukawa K, Maeshima E, Kida Y. A case of polymyositis with a significantly high level of KL-6 associated with pancreatic cancer. Mod Rheumatol. 2007;17 (3):262-4
• Kobayashi J, Kitamura S. KL-6: a serum marker for interstitial pneumonia. Chest. 1995 Aug;108 (2):311-5
• Kobayashi J, Kitamura S. Serum KL-6 for the evaluation of active pneumonitis in pulmonary sarcoidosis. Chest. 1996 May;109 (5):1276-82
• Kohno N, Kyoizumi S, Awaya Y, Fukuhara H, Yamakido M, Akiyama M. New serum indicator of interstitial pneumonitis activity. Sialylated carbohydrate antigen KL-6. Chest. 1989 Jul;96 (1):68-73
• Kumada H, Kobayashi M, Kawamura Y, Yatsuji H, Hosaka T, Sezaki H, Akuta N, Suzuki F, Kobayashi M, Suzuki Y, Ikeda K, Arase Y. Serum KL-6 level is elevated in chronic hepatitis C patients with combination therapy of pegylated interferon and ribavirin. Intern Med. 2007;46 (15):1155-60
• Kunitake R, Kuwano K, Yoshida K, Maeyama T, Kawasaki M, Hagimoto N, Hara N. KL-6, surfactant protein A and D in bronchoalveolar lavage fluid from patients with pulmonary sarcoidosis. Respiration. 2001;68 (5):488-95
• Lee GH, Ueda N, Kobayashi M, Yoshinouchi T, Fujita J, Nakanishi N, Ohtsuki Y, Furihata M. Immunohistochemical distribution of SP-D, compared with that of SP-A and KL-6, in interstitial pneumonias. Med Mol Morphol. 2007 Sep;40 (3):163-7
• Makuuchi M, Nakata M, Sugawara Y, Kokudo N, Gai R, Midorikawa Y, Seyama Y, Inagaki Y, Qu X, Guo Q, Tang W. KL-6 mucin is a useful immunohistochemical marker for cholangiocarcinoma. Oncol Rep. 2007 Apr;17 (4):737-41
• Ohtsuka M, Ishii Y, Kikuchi N. Serum KL-6 levels and acute lung inflammation. Intern Med. 2007;46 (6):335; author reply 337
• Tanimoto M, Date H, Kato K, Kanehiro A, Tanimoto Y, Soh J, Takigawa N, Tabata M, Hotta K, Toyooka S, Kiura K, Fujiwara Y. Elevated serum level of sialylated glycoprotein KL-6 predicts a poor prognosis in patients with non-small cell lung cancer treated with gefitinib. Lung Cancer. 2008 Jan;59 (1):81-7
• Yamakido M, Kobuke K, Hakoda M, Kyoizumi S, Akiyama M, Kohno N. Detection of soluble tumor-associated antigens in sera and effusions using novel monoclonal antibodies, KL-3 and KL-6, against lung adenocarcinoma. Jpn J Clin Oncol. 1988 Sep;18 (3):203-16

By research topic:

• Immune Response, Infection and Inflammation

By molecule:

• KL-6