Leptin Human ELISA, Clinical Range

Summary

Leptin, the product of the ob (obese) gene, is a single-chain 16 kDa proteohormone consisting of 146 amino acid residues. Leptin is produced by differentiated adiocytes, although production have been demonstrated in other tissues, such as fundus of the stomach, the sceletal muscle, the liver, and the placenta. Leptin is considered to play an important role in appetite control, fat metabolism and body weight regulation. It targets the central nervous system, in particular the hypothalamus, suppressing food intake and stimulating energy expenditure. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action; it decreases the appetite and increases energy consumption, causing more fat to be burned.

Features

• European Union: for in vitro diagnostic use
• Rest of the world: for research use only!
• The total assay time is less than 2.5 hours
• The kit measures total leptin
• Assay format is 96 wells
• Quality Controls are human serum based
• Standards are recombinant protein based
• Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Energy metabolism and body weight regulation, Reproduction

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum

Sample requirements

35 µl/well

Storage/Shipping

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration range

1 – 50 ng/ml

Limit of detection

0.2 ng/ml

Intra-assay (Within-Run, n=8)

CV = 5.9 %

Inter-assay (Run-to-Run, n=6)

CV = 5.6 %

Spiking Recovery

92.2 %

Dilution Linearity

100.8 %

Cross-Reactivity

References to this product

• Adam JA, Menheere PP, van Dielen FM, Soeters PB, Buurman WA, Greve JW. Decreased plasma orexin-A levels in obese individuals. Int J Obes Relat Metab Disord . 26(2):274-6 (2002)
• Arnold T, Brandlhofer S, Vrtikapa K, Stangl H, Hermann M, Zwiauer K, Mangge H, Karwautz A, Huemer J, Koller D, Schneider WJ, Strobl W. Effect of obesity on plasma clusterin: a proposed modulator of leptin action. Pediatr Res. 2011 Mar;69 (3):237-42
• Aydin HI, Yozgat Y, Demirkaya E, Olgun A, Okutan V, Lenk MK, Ozcan O. Correlation between vascular endothelial growth factor and leptin in children with cyanotic congenital heart disease. Turk J Pediatr. 2007 Oct-Dec;49 (4):360-4
• Azzoni L, Crowther NJ, Firnhaber C, Foulkes AS, Yin X, Glencross D, Gross R, Kaplan MD, Papasavvas E, Schulze D, Stevens W, van der Merwe T, Waisberg R, Sanne I, Montaner LJ. Association between HIV replication and serum leptin levels: an observational study of a cohort of HIV-1-infected South African women. J Int AIDS Soc. 2010;13 (1):33
• Bartek J, Stejskal D, Stejskal P, Oral I. Concentration of soluble leptin receptor in population. Acta Univ. Palacki. Olomuc.Fac. Med. Volume 144 (2000)
• Ben Yahia R, Lichnovska R, Gwozdiewiczova S, Kuzmina G, Chlup R, Luza J, Karpisek M, Brychta T, Petrek J. Fatty Acid Binding Proteins (FABPS) in relation to significant metabolic syndrome markers in diabetic patients and non-diabetic persons - pilot study. Int J Obesity . 29: S1-S19
• Bronsky J, Karpisek M, Bronska E, Pechova M, Jancikova B, Kotolova H, Stejskal D, Prusa R, Nevoral J. Adiponectin, adipocyte fatty acid binding protein, and epidermal fatty acid binding protein: proteins newly identified in human breast milk. Clin Chem . Sep;52(9):1763-70 (2006)
• Bronsky J, Mitrova K, Karpisek M, Mazoch J, Durilova M, Fisarkova B, Stechova K, Prusa R, Nevoral J. Adiponectin, AFABP, and leptin in human breast milk during 12 months of lactation. J Pediatr Gastroenterol Nutr. 2011 Apr;52 (4):474-7
• Cagnacci A, Arangino S, Caretto S, Mazza V, Volpe A. Sexual dimorphism in the levels of amniotic fluid leptin in pregnancies at 16 weeks of gestation: relation to fetal growth. Eur J Obstet Gynecol Reprod Biol . Jan 1;124(1):53-7 (2006)
• Cambuli VM, Musiu MC, Incani M, Paderi M, Serpe R, Marras V, Cossu E, Cavallo MG, Mariotti S, Loche S, Baroni MG. Assessment of adiponectin and leptin as biomarkers of positive metabolic outcomes after lifestyle intervention in overweight and obese children. J Clin Endocrinol Metab. 2008 Aug;93 (8):3051-7
• Capel F, Klimcakova E, Viguerie N, Roussel B, Vitkova M, Kovacikova M, Polak J, Kovacova Z, Galitzky J, Maoret JJ, Hanacek J, Pers TH, Bouloumie A, Stich V, Langin D. Macrophages and adipocytes in human obesity: adipose tissue gene expression and insulin sensitivity during calorie restriction and weight stabilization. Diabetes. 2009 Jul;58 (7):1558-67
• Creutzberg EC, Wouters EF, Vanderhoven-Augustin IM, Dentener MA, Schols AM. Disturbances in leptin metabolism are related to energy imbalance during acute exacerbations of chronic obstructive pulmonary disease. Am J Respir Crit Care Med . Oct;162(4 Pt 1):1239-45 (2000)
• Creutzberg EC, Wouters EF, Vanderhoven-Augustin IM, Dentener MA, Schols AM. Disturbances in leptin metabolism are related to energy imbalance during acute exacerbations of chronic obstructive pulmonary disease. Am J Respir Crit Care Med . 162(4 Pt 1):1239-45 (2000)
• Diez ML, Santolaria F, Tejera A, Aleman MR, Gonzalez-Reimers E, Milena A, de la Vega MJ, Martinez-Riera A. Serum leptin levels in community acquired pneumonia (CAP) are related to nutritional status and not to acute phase reaction. Cytokine. 2008 May;42 (2):156-60
• Ding Q, Mracek T, Gonzalez-Muniesa P, Kos K, Wilding J, Trayhurn P, Bing C. Identification of macrophage inhibitory cytokine-1 in adipose tissue and its secretion as an adipokine by human adipocytes. Endocrinology. 2009 Apr;150 (4):1688-96
• Dolinkova M, Dostalova I, Lacinova Z, Michalsky D, Haluzikova D, Mraz M, Kasalicky M, Haluzik M. The endocrine profile of subcutaneous and visceral adipose tissue of obese patients. Mol Cell Endocrinol. 2008 Sep 10;291 (1-2):63-70
• Dostalova I, Kavalkova P, Haluzikova D, Lacinova Z, Mraz M, Papezova H, Haluzik M. Plasma concentrations of fibroblast growth factors 19 and 21 in patients with anorexia nervosa. J Clin Endocrinol Metab. 2008 Jun 17;
• Durovcova V, Marek J, Hana V, Matoulek M, Zikan V, Haluzikova D, Kavalkova P, Lacinova Z, Krsek M, Haluzik M. Plasma concentrations of adipocyte fatty acid binding protein in patients with Cushing's syndrome. Physiol Res. 2010;59 (6):963-71
• Fernandez-Rodriguez CM, Lopez-Serrano P, Alonso S, Gutierrez ML, Lledo JL, Perez-Calle JL, Temino R, Cacho G, Nevado M, Casas ML, Gasalla JM, Bonet B. Long-term reversal of hypocholesterolaemia in patients with chronic hepatitis C is related to sustained viral response and viral genotype. Aliment Pharmacol Ther . Aug 1;24(3):507-12 (2006)
• Florentin M, Liberopoulos EN, Tellis CC, Derdemezis CS, Elisaf M, Tselepis A. Effects of Rimonabant, as Monotherapy and in Combination With Fenofibrate or Ezetimibe, on Plasma Adipokine Levels: A Pilot Study. Angiology. 2009 Nov 18;
• Giapros V, Evagelidou E, Challa A, Kiortsis D, Drougia A, Andronikou S. Serum adiponectin and leptin levels and insulin resistance in children born large for gestational age are affected by the degree of overweight. Clin Endocrinol (Oxf) . Mar;66(3):353-9 (2007)
• Haluzik M, Fiedler J, Nedvidkova J, Ceska R. Serum leptin levels in patients with hyperlipidemias. Nutrition . 16(6):429-33 (2000)
• Haluzik M, Markova M, Slaby JJ, Jiskra J, Krizova J, Hass T. The changes of serum leptin and soluble leptin receptor levels in patients undergoing mobilization of peripheral blood stem cells before autologous stem cells transplantation. Endocr Res . Aug;28(3):189-97 (2002)
• Haluzik M, Matoulek M, Svacina S, Hilgertova J, Haas T. The influence of short-term fasting on serum leptin levels, and selected hormonal and metabolic parameters in morbidly obese and lean females. Endocr Res Feb-May;27(1-2):251-60 (2001)
• Haluzik M, Sindelka G, Widimsky J Jr, Prazny M, Zelinka T, Skrha J. Serum leptin levels in patients with primary hyperaldosteronism before and after treatment: relationships to insulin sensitivity. J Hum Hypertens . 16(1):41-5 (2002)
• Haluzik M, Svobodova J, Nedvidkova J, Papezova H, Kotrlikova E, Kabrt J. The relation of serum leptin levels and the degree of protein catabolism in patients with malnutrition.
• Haluzikova D, Dostalova I, Kavalkova P, Roubicek T, Mraz M, Papezova H, Haluzik M. Serum concentrations of adipocyte fatty acid binding protein in patients with anorexia nervosa. Physiol Res. 2009;58 (4):577-81
• Hancke K, Grubeck D, Hauser N, Kreienberg R, Weiss JM. Adipocyte fatty acid-binding protein as a novel prognostic factor in obese breast cancer patients. Breast Cancer Res Treat. 2010 Jan;119 (2):367-7
• Hoareau L, Ravanan P, Gonthier MP, Delarue P, Goncalves J, Cesari M, Festy F, Roche R. Effect of PEA on LPS inflammatory action in human adipocytes. Cytokine . Jun;34(5-6):291-6 (2006)
• Hoefle G, Saely CH, Risch L, Rein P, Koch L, Schmid F, Aczel S, Marte T, Langer P, Drexel H. Leptin, leptin soluble receptor and coronary atherosclerosis. Eur J Clin Invest . Aug;37(8):629-36 (2007)
• Housova J, Wilczek H, Haluzik MM, Kremen J, Krizova J, Haluzik M. Adipocyte-derived hormones in heroin addicts: the influence of methadone maintenance treatment. Physiol Res . 54(1):73-8 (2005)
• Hrebicek J, Janout V, Malincikova J, Horakova D, Cizek L. Detection of insulin resistance by simple quantitative insulin sensitivity check index QUICKI for epidemiological assessment and prevention. J Hum Hypertens . Jan;87(1):144-7 (2002)
• Hulejova H, Levitova A, Kuklova M, Stochl J, Haluzik M, Pavelka K, Vencovsky J, Senolt L. No effect of physiotherapy on the serum levels of adipocytokines in patients with ankylosing spondylitis. Clin Rheumatol. 2011 May 28;
• Jarkovska Z, Hodkova M, Sazamova M, Rosicka M, Dusilova-Sulkova S, Marek J, Justova V, Lacinova Z, Haluzik M, Haas T, Krsek M. Plasma levels of active and total ghrelin in renal failure: a relationship with GH/IGF-I axis. Growth Horm IGF Res . Dec;15(6):369-76 (2005)
• Kalman S, Buyan N, Bayazit AK, Bideci A, Bakkaloglu S, ozkaya O, Soylemezoglu O. The role of leptin and soluble leptin receptor on the nutritional parameters in pediatric CPD patients. Nephrology Dialysis Transplantation . Volume 18 Supplement 4 June 2003
• Kalousova M, Zima T, Popov P, Spacek P, Braun M, Soukupova J, Pelinkova K, Kientsch-Engel R. Advanced glycation end-products in patients with chronic alcohol misuse. Alcohol Alcohol . Jul-Aug;39(4):316-20 (2004)
• Karapanagiotou EM, Tsochatzis EA, Dilana KD, Tourkantonis I, Gratsias I, Syrigos KN. The significance of leptin, adiponectin, and resistin serum levels in non-small cell lung cancer (NSCLC). Lung Cancer. 2008 Mar 12;
• Koch A, Weiskirchen R, Zimmermann HW, Sanson E, Trautwein C, Tacke F. Relevance of serum leptin and leptin-receptor concentrations in critically ill patients. Mediators Inflamm. 2010;2010
• Krizova J, Sulkova S, Bednarova V, Parizkova J, Kotrlikova E, Haluzik M. Soluble leptin receptor levels in patients with chronic renal failure. Physiol Res . 52(3):347-351 (2003)
• Krsek M, Rosicka M, Haluzik M, Svobodova J, Kotrlikova E, Justova V, Lacinova Z, Jarkovska Z. Plasma ghrelin levels in patients with short bowel syndrome. Endocr Res . Feb-May;28(1-2):27-33 (2002)
• Krsek M, Silha JV, Jezkova J, Hana V, Marek J, Weiss V, Stepan JJ, Murphy LJ. Adipokine levels in Cushing's syndrome; elevated resistin levels in female patients with Cushing's syndrome. Clin Endocrinol (Oxf) . Mar;60(3):350-7 (2004)
• Kukla M, Zwirska-Korczala K, Gabriel A, Waluga M, Warakomska I, Szczygiel B, Berdowska A, Mazur W, Wozniak-Grygiel E, Kryczka W. Chemerin, vaspin and insulin resistance in chronic hepatitis C. J Viral Hepat. 2010 Sep;17 (9):661-7
• Lamota L, Bermudez-Silva FJ, Marco EM, Llorente R, Gallego A, Rodriguez de Fonseca F, Viveros MP. Effects of adolescent nicotine and SR 147778 (Surinabant) administration on food intake, somatic growth and metabolic parameters in rats. Neuropharmacology. 2008 Jan;54 (1):194-205
• Lichnovska R, Gwozdziewiczova S, Hrebicek J. Gender differences in factors influencing insulin resistance in elderly hyperlipemic non-diabetic subjects. Cardiovasc Diabetol . Oct 14;1(1):4 (2002)
• Lin SY, Huang SC, Sheu WH. Circulating adiponectin concentrations were related to free thyroxine levels in thyroid cancer patients after thyroid hormone withdrawal. Metabolism. 2010 Feb;59 (2):195-9
• Maletinska L, Toma RS, Pirnik Z, Kiss A, Slaninova J, Haluzik M, Zelezna B. Effect of cholecystokinin on feeding is attenuated in monosodium glutamate obese mice. Regul Pept . Sep 11;136(1-3):58-63 (2006)
• Malincikova J, Stejskal D, Hrebicek J. Serum leptin and leptin receptors in healthy prepubertal children: relations to insulin resistance and lipid parameters, body mass index (BMI), tumor necrosis factor alpha (TNF alpha), heart fatty acid binding protein (hFABP), and IgG anticardiolipin. Acta Univ Palacki Olomuc Fac Med . 143:51-7 (2000)
• Mangge H, Freytag F, Almer G, Weghuber D, Bauer-Denk C, Fuchs D. Serum neopterin is not increased in obese juveniles. J Obes. 2011;2011:946795
• Markova M, Haluzik M, Svobodova J, Rosicka M, Nedvidkova J, Haas T. Serum leptin levels in patients with sideropenic and pernicious anemia: the influence of anemia treatment. Physiol Res. 2000;49 (6):679-84
• Mazur W, Kukla M, Logiewa-Bazger B, Beniowski M, Kwiatkowski J, Wagner G, Jurzak M, Berdowska A, Żwirska-Korczala K. Resistin, leptin and ghrelin serum levels among the patients with chronic hepatitis C. E&C Hepatology. 2010;6(3-4):17-24
• Meier U, Gressner AM. Endocrine regulation of energy metabolism: review of pathobiochemical and clinical chemical aspects of leptin, ghrelin, adiponectin, and resistin. Clin Chem. 2004 Sep;50 (9):1511-25
• Mente A, Razak F, Blankenberg S, Vuksan V, Davis AD, Miller R, Teo K, Gerstein H, Sharma AM, Yusuf S, Anand SS. Ethnic variation in adiponectin and leptin levels and their association with adiposity and insulin resistance. Diabetes Care. 2010 Jul;33 (7):1629-34
• Mitchell SE, Nogueiras R, Morris A, Tovar S, Grant C, Cruickshank M, Rayner DV, Dieguez C, Williams LM. Leptin receptor gene expression and number in the brain are regulated by leptin level and nutritional status. J Physiol. 2009 Jul 15;587 (Pt 14):3573-85
• Moro C, Klimcakova E, Lolmede K, Berlan M, Lafontan M, Stich V, Bouloumie A, Galitzky J, Arner P, Langin D. Atrial natriuretic peptide inhibits the production of adipokines and cytokines linked to inflammation and insulin resistance in human subcutaneous adipose tissue. Diabetologia . May;50(5):1038-47 (2007)
• Olszanecka-Glinianowicz M, Zahorska-Markiewicz B, Kocelak P, Janowska J, Semik-Grabarczyk E, Wikarek T, Gruszka W, Dabrowski P. Is chronic inflammation a possible cause of obesity-related depression?. Mediators Inflamm. 2009;2009:439107
• Pamuk GE, Demir M, Harmandar F, Yesil Y, Turgut B, Vural O. Leptin and resistin levels in serum of patients with hematologic malignancies: correlation with clinical characteristics. Exp Oncol . Sep;28(3):241-4 (2006)
• Paul G, Schaffler A, Neumeier M, Furst A, Bataillle F, Buechler C, Muller-Ladner U, Scholmerich J, Rogler G, Herfarth H. Profiling adipocytokine secretion from creeping fat in Crohn's disease. Inflamm Bowel Dis . Jun;12(6):471-7 (2006)
• Petzel M, Stejskal D, Jedelsky L, Kadalova L, Safarcik K. The influence of estradiole and tibolone administration on leptin levels in women with surgically induced menopause. Biomed Pap Med Fac Univ Palack. 2008 Jun;152 (1):101-5
• Pilz S, Horejsi R, Moller R, Almer G, Scharnagl H, Stojakovic T, Dimitrova R, Weihrauch G, Borkenstein M, Maerz W, Schauenstein K, Mangge H. Early atherosclerosis in obese juveniles is associated with low serum levels of adiponectin. J Clin Endocrinol Metab . Aug;90(8):4792-6 (2005)
• Popa M, Stefanescu AM, Procopiuc C, Dumitrescu C. Serum Leptin and Leptin binding activity after ten days administration of rhGH (as IGF I generation test) in short children. Endocrine Care. 2007;vol. III (no. 2, p.):p. 161-168
• Ripolles Piquer B, Nazih H, Bourreille A, Segain JP, Huvelin JM, Galmiche JP, Bard JM. Altered lipid, apolipoprotein, and lipoprotein profiles in inflammatory bowel disease: consequences on the cholesterol efflux capacity of serum using Fu5AH cell system. Metabolism . Jul;55(7):980-8 (2006)
• Risch L, Saely C, Hoefle G, Rein P, Langer P, Gouya G, Marte T, Aczel S, Drexel H. Relationship between glomerular filtration rate and the adipokines adiponectin, resistin and leptin in coronary patients with predominantly normal or mildly impaired renal function. Clin Chim Acta. 2007 Feb;376 (1-2):108-13
• Ruffingshofer D, Endemann M, Arbeiter K, Bidmon B, Mueller T, Herkner K, Aufricht C. Induction of heat shock protein 72 in mesothelial cells exposed to peritoneal dialysate effluent. Perit Dial Int. 2003 Jan-Feb;23 (1):74-7
• Sainz N, Rodriguez A, Catalan V, Becerril S, Ramirez B, Gomez-Ambrosi J, Fruhbeck G. Leptin administration favors muscle mass accretion by decreasing FoxO3a and increasing PGC-1alpha in ob/ob mice. PLoS One. 2009;4 (9):e6808
• Schaffler A, Landfried K, Volk M, Furst A, Buchler C, Scholmerich J, Herfarth H. Potential of adipocytokines in predicting peripancreatic necrosis and severity in acute pancreatitis: pilot study. J Gastroenterol Hepatol. 2007 Mar;22 (3):326-34
• Semnani S, Roshandel G, Keshtkar A, Najafi L, Amiriani T, Farajollahi M, Moradi A, Joshaghani H. Serum leptin levels and irritable bowel syndrome: a new hypothesis. J Clin Gastroenterol. 2009 Oct;43 (9):826-30
• Senolt L, Kuklova M, Cerezo LA, Hulejova H, Filkova M, Bosanska L, Pecha O, Pavelka K, Haluzik M, Vencovsky J. Adipokine profile is modulated in subcutaneous adipose tissue by TNF{alpha} inhibitors in patients with rheumatoid arthritis. Ann Rheum Dis. 2011 Nov;70 (11):2054-6
• Silha JV, Nyomba BL, Leslie WD, Murphy LJ. Ethnicity, insulin resistance, and inflammatory adipokines in women at high and low risk for vascular disease. Diabetes Care. 2007 Feb;30 (2):286-91
• Stejskal D, Adamovska S, Bartek J, Jurakova R, Proskova J. Resistin - concentrations in persons with type 2 diabetes mellitus and in individuals with acute inflammatory disease. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub . Nov;147(1):63-9 (2003)
• Stejskal D, Bartek J, Ruzicka V, Stejskal P, Jedelsky L, Oral I, Horalik D, Zurek M. Determination of leptin receptor in the serum and relations to laboratory and anthropological parameters in patients with atherosclerotic complications. Acta Univ Palacki Olomouc Fac Med . 142:89-92 (1999)
• Stejskal D, Ruzicka V, Adamovska S, Jurakova R, Proskova J, Jedelsky L, Bartek J. Adiponectin concentrations as a criterion of metabolic control in persons with type 2 diabetes mellitus?. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub . Dec;147(2):167-72 (2003)
• Stejskal D, Stejskal P, Ruzicka V, Jedelsky L, Horalik D, Kalina M, Bartek J. Leptinaemia in the Czech population. Vnitr Lek . Mar;45(3):139-47 (1999)
• Teplan V, Schuck O, Stollova M, Vitko S. Obesity and hyperhomocysteinaemia after kidney transplantation. Nephrol Dial Transplant . Jul;18 Suppl 5:v71-3 (2003)
• van Dielen FM, van 't Veer C, Buurman WA, Greve JW. Leptin and soluble leptin receptor levels in obese and weight-losing individuals. J Clin Endocrinol Metab. 2002 Apr;87 (4):1708-16
• van Dielen FM, van't Veer C, Schols AM, Soeters PB, Buurman WA, Greve JW. Increased leptin concentrations correlate with increased concentrations of inflammatory markers in morbidly obese individuals. Int J Obes Relat Metab Disord . 25(12):1759-66 (2001)
• Vitkova M, Klimcakova E, Kovacikova M, Valle C, Moro C, Polak J, Hanacek J, Capel F, Viguerie N, Richterova B, Bajzova M, Hejnova J, Stich V, Langin D. Plasma levels and adipose tissue messenger ribonucleic acid expression of retinol-binding protein 4 are reduced during calorie restriction in obese subjects but are not related to diet-induced changes in insulin sensitivity. J Clin Endocrinol Metab . Jun;92(6):2330-5 (2007)
• Welt CK, Schneyer AL, Heist K, Mantzoros CS. Leptin and soluble leptin receptor in follicular fluid. J Assist Reprod Genet. Dec;20(12):495-501 (2003)
• Zarei S, Soltanghoraee H, Mohammadzadeh A, Arefi S, Zarnani AH, Idali. Serum Leptin Levels in Women with Immunological Recurrent Abortion. J Reprod Infertil. 2010;11(1);:47-52

References to summary

• Auwerx J. and Staels B.: Leptin (Review article). The Lancet 13, 737 (1998)
• Blum W.F., Englaro P., Hanitsch S. et al.: Plasma leptin levels in healthy children and adolescents: Dependence on body mass index, body fat mass, gender, pubertal stage, and testosterone. J. Clin. Endocrinol. Metab. 82, 2904 (1997)
• Cohen B., Novick D. and Rubinstein M.: Modulation of insulin activities by leptin. Science 274, 1185 (1996)
• Considine R.V., Sinha M.K., Heiman M.L., Kriaciunas A., Stephens T.W., Nyce M.R., Ohannesian J.P., Marco C.C., McKee L.J., Bauer T.L. and Caro J.F.: Serum immunoreactive leptin concentrations in normal-weight and obese humans. N. Engl. J. Med. 334, 292–295 (1996)
• Halaas J.L., Gajiwala K.S., Maffei M., Cohen S.L., Chait B.T., Rabinowitz D., Lallone R.L., Burley S.K. and Friedman J.M.: Weight-reducing effects of the plasma protein encoded by the obese gene. Science 269, 543–546 (1995)
• Harigaya A., Nagashima K., Nako Y. and Marikawa A.: Relationship between concentration of serum leptin and fetal growth. J. Clin. Endocrinol. Metab. 82, 3281 (1997)
• Lönnqvist F., Arner P., Nordfors L. and Shalling M.: Overexpression of the obese (ob) gene in adipose tissue of human subjects. Nature Med. 1, 950–953 (1995)
• Maffei M., Halaas J., Ravussin E., Pratley R.E., Lee G.H., Zhang Y., Fei H., Kim S., Lallone R., Ranganathan S., Kern P.A. and Friedman J.M.: Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nature Med. 1, 1155–1161 (1995)
• Pelleymounter M.A., Cullen M.J., Baker M.B., Hecht R., Winters D., Boone T. and Collins F.: Effects of the obese gene product on body weight regulation in ob/ob mice. Science 269, 540–543 (1995)
• Spiegelman B.M., Flier J.S.: Adipogenesis and obesity: Rounding out the big picture, Cell 87, 377–389 (1996)
• Tartaglia L.A.: The leptin receptor, J. Biol. Chem. 272, 6093–6096 (1997)
• Tritos N.A., Mantzoros C.S.: Leptin: its role in obesity and beyond, Diabetologia 40, 1371–1379 (1997)
• Zhang F., Basinski M. B., Beals J.M., Briggs S.L., Churgay L.M., Clawson D.K., DiMarchi R.D., Furman T.C., Hale J.E., Hsiung H.M., Schoner B.E., Smith D.P., Zhang X.Y., Wery J.P., and Schevitz R.W.: Crystal structure of the obese protein leptin-E100, Nature 387, 206–209 (1997)
• Zhang Y., Proenca R., Maffei M., Barone M., Leopold L., Friedman J.M.: Positional cloning of the mouse obese gene and its human homologue. Nature 372, 425–432 (1994)

By research topic:

• Energy metabolism and body weight regulation
• Reproduction

By molecule:

• Leptin

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