Leptin Human ELISA, Clinical Range

Summary

Leptin, the product of the ob (obese) gene, is a single-chain 16 kDa proteohormone consisting of 146 amino acid residues. Leptin is produced by differentiated adiocytes, although production have been demonstrated in other tissues, such as fundus of the stomach, the sceletal muscle, the liver, and the placenta. Leptin is considered to play an important role in appetite control, fat metabolism and body weight regulation. It targets the central nervous system, in particular the hypothalamus, suppressing food intake and stimulating energy expenditure. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action; it decreases the appetite and increases energy consumption, causing more fat to be burned.

Features

• European Union: for in vitro diagnostic use
• Rest of the world: for research use only!
• The total assay time is less than 2.5 hours
• The kit measures total leptin
• Assay format is 96 wells
• Quality Controls are human serum based
• Standards are recombinant protein based
• Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Energy metabolism and body weight regulation, Reproduction

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum

Sample requirements

35 µl/well

Storage/Shipping

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration range

1 – 50 ng/ml

Limit of detection

0.2 ng/ml

Intra-assay (Within-Run, n=8)

CV = 5.9 %

Inter-assay (Run-to-Run, n=6)

CV = 5.6 %

Spiking Recovery

92.2 %

Dilution Linearity

100.8 %

Cross-Reactivity

References to this product

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References to summary

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By research topic:

• Energy metabolism and body weight regulation
• Reproduction

By molecule:

• Leptin

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