Leptin Human ELISA, Clinical Range

Research topic

Animal studies, Energy metabolism and body weight regulation, Reproduction

Features

• The total assay time is less than four hours.
• The kit measures total serum leptin.
• Quality controls are human serum based. No animal sera are used.
• Components of the kit are ready-to-use (with the exception of Wash Solution and Quality Controls).

Storage/Shipping

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Summary

Leptin, the product of the ob (obese) gene, is a single-chain 16 kDa proteohormone consisting of 146 amino acid residues. Leptin is produced by differentiated adiocytes, although production have been demonstrated in other tissues, such as fundus of the stomach, the sceletal muscle, the liver, and the placenta. Leptin is considered to play an important role in appetite control, fat metabolism and body weight regulation. It targets the central nervous system, in particular the hypothalamus, suppressing food intake and stimulating energy expenditure. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action; it decreases the appetite and increases energy consumption, causing more fat to be burned.

Assay format

Sandwich ELISA, HRP-labelled antibody

Sample requirements

35 µl/well

Applications

Cell culture medium, Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum

Calibration Curve

Limit of detection

Analytical Limit of Detection is calculated from the real leptin values in wells and is 0.17ng/ml

Limit of quantification

Assay Sensitivity takes the dilution of samples into consideration and is calculated according to the formula: Assay Sensitivity = Analytical Limit of Detection x sample dilution = 0.17ng/ml x 3 = 0.5 ng/ml

Intra-assay (Within-Run, n=8)

CV = 5.7 %

Inter-assay (Run-to-Run, n=6)

CV = 6.8 %

Spiking Recovery

90.8 %

Dilution Linearity

105.3 %

Cross-Reactivity

Human, Dog, Goat

References to this product

• Housova J, Wilczek H, Haluzik MM, Kremen J, Krizova J, Haluzik M . Adipocyte-derived hormones in heroin addicts: the influence of methadone maintenance treatment. Physiol Res . 54(1):73-8 (2005)
• Krsek M, Silha JV, Jezkova J, Hana V, Marek J, Weiss V, Stepan JJ, Murphy LJ . Adipokine levels in Cushing's syndrome; elevated resistin levels in female patients with Cushing's syndrome. Clin Endocrinol (Oxf) . Mar;60(3):350-7 (2004)
• Stejskal D, Ruzicka V, Adamovska S, Jurakova R, Proskova J, Jedelsky L, Bartek J . Adiponectin concentrations as a criterion of metabolic control in persons with type 2 diabetes mellitus?. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub . Dec;147(2):167-72 (2003)
• Bronsky J, Karpisek M, Bronska E, Pechova M, Jancikova B, Kotolova H, Stejskal D, Prusa R, Nevoral J . Adiponectin, adipocyte fatty acid binding protein, and epidermal fatty acid binding protein: proteins newly identified in human breast milk. Clin Chem . Sep;52(9):1763-70 (2006)
• Kalousova M, Zima T, Popov P, Spacek P, Braun M, Soukupova J, Pelinkova K, Kientsch-Engel R . Advanced glycation end-products in patients with chronic alcohol misuse. Alcohol Alcohol . Jul-Aug;39(4):316-20 (2004)
• Ripolles Piquer B, Nazih H, Bourreille A, Segain JP, Huvelin JM, Galmiche JP, Bard JM . Altered lipid, apolipoprotein, and lipoprotein profiles in inflammatory bowel disease: consequences on the cholesterol efflux capacity of serum using Fu5AH cell system. Metabolism . Jul;55(7):980-8 (2006)
• Moro C, Klimcakova E, Lolmede K, Berlan M, Lafontan M, Stich V, Bouloumie A, Galitzky J, Arner P, Langin D . Atrial natriuretic peptide inhibits the production of adipokines and cytokines linked to inflammation and insulin resistance in human subcutaneous adipose tissue. Diabetologia . May;50(5):1038-47 (2007)
• Bartek J, Stejskal D, Stejskal P, Oral I . Concentration of soluble leptin receptor in population. Acta Univ. Palacki. Olomuc.Fac. Med. Volume 144 (2000)
• Adam JA, Menheere PP, van Dielen FM, Soeters PB, Buurman WA, Greve JW . Decreased plasma orexin-A levels in obese individuals. Int J Obes Relat Metab Disord . 26(2):274-6 (2002)
• Hrebicek J, Janout V, Malincikova J, Horakova D, Cizek L. Detection of insulin resistance by simple quantitative insulin sensitivity check index QUICKI for epidemiological assessment and prevention. J Hum Hypertens . Jan;87(1):144-7 (2002)
• Stejskal D, Bartek J, Ruzicka V, Stejskal P, Jedelsky L, Oral I, Horalik D, Zurek M . Determination of leptin receptor in the serum and relations to laboratory and anthropological parameters in patients with atherosclerotic complications. Acta Univ Palacki Olomouc Fac Med . 142:89-92 (1999)
• Creutzberg EC, Wouters EF, Vanderhoven-Augustin IM, Dentener MA, Schols AM . Disturbances in leptin metabolism are related to energy imbalance during acute exacerbations of chronic obstructive pulmonary disease. Am J Respir Crit Care Med . Oct;162(4 Pt 1):1239-45 (2000)
• Creutzberg EC, Wouters EF, Vanderhoven-Augustin IM, Dentener MA, Schols AM . Disturbances in leptin metabolism are related to energy imbalance during acute exacerbations of chronic obstructive pulmonary disease. Am J Respir Crit Care Med . 162(4 Pt 1):1239-45 (2000)
• Pilz S, Horejsi R, Moller R, Almer G, Scharnagl H, Stojakovic T, Dimitrova R, Weihrauch G, Borkenstein M, Maerz W, Schauenstein K, Mangge H . Early atherosclerosis in obese juveniles is associated with low serum levels of adiponectin. J Clin Endocrinol Metab . Aug;90(8):4792-6 (2005)
• Maletinska L, Toma RS, Pirnik Z, Kiss A, Slaninova J, Haluzik M, Zelezna B . Effect of cholecystokinin on feeding is attenuated in monosodium glutamate obese mice. Regul Pept . Sep 11;136(1-3):58-63 (2006)
• Hoareau L, Ravanan P, Gonthier MP, Delarue P, Goncalves J, Cesari M, Festy F, Roche R . Effect of PEA on LPS inflammatory action in human adipocytes. Cytokine . Jun;34(5-6):291-6 (2006)
• Lamota L, Bermudez-Silva FJ, Marco EM, Llorente R, Gallego A, Rodriguez de Fonseca F, Viveros MP. Effects of adolescent nicotine and SR 147778 (Surinabant) administration on food intake, somatic growth and metabolic parameters in rats. Neuropharmacology. 2008 Jan;54 (1):194-205
• Ben Yahia R, Lichnovska R, Gwozdiewiczova S, Kuzmina G, Chlup R, Luza J, Karpisek M, Brychta T, Petrek J . Fatty Acid Binding Proteins (FABPS) in relation to significant metabolic syndrome markers in diabetic patients and non-diabetic persons - pilot study. Int J Obesity . 29: S1-S19
• Lichnovska R, Gwozdziewiczova S, Hrebicek J . Gender differences in factors influencing insulin resistance in elderly hyperlipemic non-diabetic subjects. Cardiovasc Diabetol . Oct 14;1(1):4 (2002)
• van Dielen FM, van't Veer C, Schols AM, Soeters PB, Buurman WA, Greve JW . Increased leptin concentrations correlate with increased concentrations of inflammatory markers in morbidly obese individuals. Int J Obes Relat Metab Disord . 25(12):1759-66 (2001)
• Pamuk GE, Demir M, Harmandar F, Yesil Y, Turgut B, Vural O . Leptin and resistin levels in serum of patients with hematologic malignancies: correlation with clinical characteristics. Exp Oncol . Sep;28(3):241-4 (2006)
• Welt CK, Schneyer AL, Heist K, Mantzoros CS . Leptin and soluble leptin receptor in follicular fluid. J Assist Reprod Genet. Dec;20(12):495-501 (2003)
• Hoefle G, Saely CH, Risch L, Rein P, Koch L, Schmid F, Aczel S, Marte T, Langer P, Drexel H . Leptin, leptin soluble receptor and coronary atherosclerosis. Eur J Clin Invest . Aug;37(8):629-36 (2007)
• Stejskal D, Stejskal P, Ruzicka V, Jedelsky L, Horalik D, Kalina M, Bartek J . Leptinaemia in the Czech population. Vnitr Lek . Mar;45(3):139-47 (1999)
• Fernandez-Rodriguez CM, Lopez-Serrano P, Alonso S, Gutierrez ML, Lledo JL, Perez-Calle JL, Temino R, Cacho G, Nevado M, Casas ML, Gasalla JM, Bonet B . Long-term reversal of hypocholesterolaemia in patients with chronic hepatitis C is related to sustained viral response and viral genotype. Aliment Pharmacol Ther . Aug 1;24(3):507-12 (2006)
• Teplan V, Schuck O, Stollova M, Vitko S . Obesity and hyperhomocysteinaemia after kidney transplantation. Nephrol Dial Transplant . Jul;18 Suppl 5:v71-3 (2003)
• Dostalova I, Kavalkova P, Haluzikova D, Lacinova Z, Mraz M, Papezova H, Haluzik M. Plasma concentrations of fibroblast growth factors 19 and 21 in patients with anorexia nervosa. J Clin Endocrinol Metab. 2008 Jun 17;
• Krsek M, Rosicka M, Haluzik M, Svobodova J, Kotrlikova E, Justova V, Lacinova Z, Jarkovska Z . Plasma ghrelin levels in patients with short bowel syndrome. Endocr Res . Feb-May;28(1-2):27-33 (2002)
• Vitkova M, Klimcakova E, Kovacikova M, Valle C, Moro C, Polak J, Hanacek J, Capel F, Viguerie N, Richterova B, Bajzova M, Hejnova J, Stich V, Langin D . Plasma levels and adipose tissue messenger ribonucleic acid expression of retinol-binding protein 4 are reduced during calorie restriction in obese subjects but are not related to diet-induced changes in insulin sensitivity. J Clin Endocrinol Metab . Jun;92(6):2330-5 (2007)
• Jarkovska Z, Hodkova M, Sazamova M, Rosicka M, Dusilova-Sulkova S, Marek J, Justova V, Lacinova Z, Haluzik M, Haas T, Krsek M . Plasma levels of active and total ghrelin in renal failure: a relationship with GH/IGF-I axis. Growth Horm IGF Res . Dec;15(6):369-76 (2005)
• Schaffler A, Landfried K, Volk M, Furst A, Buchler C, Scholmerich J, Herfarth H. Potential of adipocytokines in predicting peripancreatic necrosis and severity in acute pancreatitis: pilot study. J Gastroenterol Hepatol. 2007 Mar;22 (3):326-34
• Paul G, Schaffler A, Neumeier M, Furst A, Bataillle F, Buechler C, Muller-Ladner U, Scholmerich J, Rogler G, Herfarth H . Profiling adipocytokine secretion from creeping fat in Crohn's disease. Inflamm Bowel Dis . Jun;12(6):471-7 (2006)
• Risch L, Saely C, Hoefle G, Rein P, Langer P, Gouya G, Marte T, Aczel S, Drexel H. Relationship between glomerular filtration rate and the adipokines adiponectin, resistin and leptin in coronary patients with predominantly normal or mildly impaired renal function. Clin Chim Acta. 2007 Feb;376 (1-2):108-13
• Stejskal D, Adamovska S, Bartek J, Jurakova R, Proskova J . Resistin - concentrations in persons with type 2 diabetes mellitus and in individuals with acute inflammatory disease. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub . Nov;147(1):63-9 (2003)
• Giapros V, Evagelidou E, Challa A, Kiortsis D, Drougia A, Andronikou S . Serum adiponectin and leptin levels and insulin resistance in children born large for gestational age are affected by the degree of overweight. Clin Endocrinol (Oxf) . Mar;66(3):353-9 (2007)
• Malincikova J, Stejskal D, Hrebicek J . Serum leptin and leptin receptors in healthy prepubertal children: relations to insulin resistance and lipid parameters, body mass index (BMI), tumor necrosis factor alpha (TNF alpha), heart fatty acid binding protein (hFABP), and IgG anticardiolipin. Acta Univ Palacki Olomuc Fac Med . 143:51-7 (2000)
• Diez ML, Santolaria F, Tejera A, Aleman MR, Gonzalez-Reimers E, Milena A, de la Vega MJ, Martinez-Riera A. Serum leptin levels in community acquired pneumonia (CAP) are related to nutritional status and not to acute phase reaction. Cytokine. 2008 May;42 (2):156-60
• Haluzik M, Fiedler J, Nedvidkova J, Ceska R. Serum leptin levels in patients with hyperlipidemias. Nutrition . 16(6):429-33 (2000)
• Haluzik M, Sindelka G, Widimsky J Jr, Prazny M, Zelinka T, Skrha J . Serum leptin levels in patients with primary hyperaldosteronism before and after treatment: relationships to insulin sensitivity. J Hum Hypertens . 16(1):41-5 (2002)
• Cagnacci A, Arangino S, Caretto S, Mazza V, Volpe A . Sexual dimorphism in the levels of amniotic fluid leptin in pregnancies at 16 weeks of gestation: relation to fetal growth. Eur J Obstet Gynecol Reprod Biol . Jan 1;124(1):53-7 (2006)
• Krizova J, Sulkova S, Bednarova V, Parizkova J, Kotrlikova E, Haluzik M . Soluble leptin receptor levels in patients with chronic renal failure. Physiol Res . 52(3):347-351 (2003)
• Haluzik M, Markova M, Slaby JJ, Jiskra J, Krizova J, Hass T . The changes of serum leptin and soluble leptin receptor levels in patients undergoing mobilization of peripheral blood stem cells before autologous stem cells transplantation. Endocr Res . Aug;28(3):189-97 (2002)
• Haluzik M, Matoulek M, Svacina S, Hilgertova J, Haas T. The influence of short-term fasting on serum leptin levels, and selected hormonal and metabolic parameters in morbidly obese and lean females. Endocr Res Feb-May;27(1-2):251-60 (2001)
• Haluzik M, Svobodova J, Nedvidkova J, Papezova H, Kotrlikova E, Kabrt J . The relation of serum leptin levels and the degree of protein catabolism in patients with malnutrition.
• Kalman S, Buyan N, Bayazit AK, Bideci A, Bakkaloglu S, ozkaya O, Soylemezoglu O . The role of leptin and soluble leptin receptor on the nutritional parameters in pediatric CPD patients. Nephrology Dialysis Transplantation . Volume 18 Supplement 4 June 2003
• Karapanagiotou EM, Tsochatzis EA, Dilana KD, Tourkantonis I, Gratsias I, Syrigos KN. The significance of leptin, adiponectin, and resistin serum levels in non-small cell lung cancer (NSCLC). Lung Cancer. 2008 Mar 12;

References to summary

• Auwerx J. and Staels B.: Leptin (Review article). The Lancet 13, 737 (1998)
• Blum W.F., Englaro P., Hanitsch S. et al.: Plasma leptin levels in healthy children and adolescents: Dependence on body mass index, body fat mass, gender, pubertal stage, and testosterone. J. Clin. Endocrinol. Metab. 82, 2904 (1997)
• Cohen B., Novick D. and Rubinstein M.: Modulation of insulin activities by leptin. Science 274, 1185 (1996)
• Considine R.V., Sinha M.K., Heiman M.L., Kriaciunas A., Stephens T.W., Nyce M.R., Ohannesian J.P., Marco C.C., McKee L.J., Bauer T.L. and Caro J.F.: Serum immunoreactive leptin concentrations in normal-weight and obese humans. N. Engl. J. Med. 334, 292–295 (1996)
• Halaas J.L., Gajiwala K.S., Maffei M., Cohen S.L., Chait B.T., Rabinowitz D., Lallone R.L., Burley S.K. and Friedman J.M.: Weight-reducing effects of the plasma protein encoded by the obese gene. Science 269, 543–546 (1995)
• Harigaya A., Nagashima K., Nako Y. and Marikawa A.: Relationship between concentration of serum leptin and fetal growth. J. Clin. Endocrinol. Metab. 82, 3281 (1997)
• Lönnqvist F., Arner P., Nordfors L. and Shalling M.: Overexpression of the obese (ob) gene in adipose tissue of human subjects. Nature Med. 1, 950–953 (1995)
• Maffei M., Halaas J., Ravussin E., Pratley R.E., Lee G.H., Zhang Y., Fei H., Kim S., Lallone R., Ranganathan S., Kern P.A. and Friedman J.M.: Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nature Med. 1, 1155–1161 (1995)
• Pelleymounter M.A., Cullen M.J., Baker M.B., Hecht R., Winters D., Boone T. and Collins F.: Effects of the obese gene product on body weight regulation in ob/ob mice. Science 269, 540–543 (1995)
• Spiegelman B.M., Flier J.S.: Adipogenesis and obesity: Rounding out the big picture, Cell 87, 377–389 (1996)
• Tartaglia L.A.: The leptin receptor, J. Biol. Chem. 272, 6093–6096 (1997)
• Tritos N.A., Mantzoros C.S.: Leptin: its role in obesity and beyond, Diabetologia 40, 1371–1379 (1997)
• Zhang F., Basinski M. B., Beals J.M., Briggs S.L., Churgay L.M., Clawson D.K., DiMarchi R.D., Furman T.C., Hale J.E., Hsiung H.M., Schoner B.E., Smith D.P., Zhang X.Y., Wery J.P., and Schevitz R.W.: Crystal structure of the obese protein leptin-E100, Nature 387, 206–209 (1997)
• Zhang Y., Proenca R., Maffei M., Barone M., Leopold L., Friedman J.M.: Positional cloning of the mouse obese gene and its human homologue. Nature 372, 425–432 (1994)