Leptin Mouse/Rat ELISA

Summary

Leptin, the product of the ob (obese) gene, is a single-chain 16 kDa proteohormone consisting of 146 amino acid residues. Leptin is produced by differentiated adiocytes, although production have been demonstrated in other tissues, such as fundus of the stomach, the sceletal muscle, the liver, and the placenta. Leptin is considered to play an important role in appetite control, fat metabolism and body weight regulation. It targets the central nervous system, in particular the hypothalamus, suppressing food intake and stimulating energy expenditure. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action; it decreases the appetite and increases energy consumption, causing more fat to be burned.

Features

• The total assay time is less than four hours.
• The kit measures total serum leptin.
• Quality controls are mouse and rat serum based. No human sera are used.

Research topic

Animal studies, Energy metabolism and body weight regulation, Reproduction

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture medium, Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum

Sample requirements

5 µl/well

Storage/Shipping

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration range

100 – 4000 pg/ml

Limit of detection

Analytical Limit of Detection is calculated from the real leptin values in wells and is 30 pg/ml for mouse leptin and 50 pg/ml for rat leptin

Intra-assay (Within-Run, n=8)

CV = 2.2 %

Inter-assay (Run-to-Run, n=8)

CV = 3.4 %

Spiking Recovery

94.5 %

Dilution Linearity

96.7 %

Cross-Reactivity

References to this product

• Bartels ED, Nielsen JM, Hellgren LI, Ploug T, Nielsen LB. Cardiac expression of microsomal triglyceride transfer protein is increased in obesity and serves to attenuate cardiac triglyceride accumulation. PLoS One. 2009;4 (4):e5300
• Bol VV, Delattre AI, Reusens B, Raes M, Remacle C. Forced catch-up growth after fetal protein restriction alters the adipose tissue gene expression program leading to obesity in adult mice. Am J Physiol Regul Integr Comp. 2009 Aug;297 (2):R291-9
• Giri S, Rattan R, Hag E, Khan M, Yasmin R, Won JS, Key L, Singh AK, Singh I. AICAR inhibits adipocyte differentiation in 3T3L1 and restores metabolic alterations in diet-induced obesity mice model. Nutr Metab (Lond) . Aug 10;3:31 (2006)
• Gout J, Sarafian D, Tirard J, Blondet A, Vigier M, Rajas F, Mithieux G, Begeot M, Naville D. Leptin infusion and obesity in mouse cause alterations in the hypothalamic melanocortin system. Obesity (Silver Spring). 2008 Aug;16 (8):1763-9
• Kirk SL, Samuelsson AM, Argenton M, Dhonye H, Kalamatianos T, Poston L, Taylor PD, Coen CW. Maternal obesity induced by diet in rats permanently influences central processes regulating food intake in offspring. PLoS One. 2009;4 (6):e5870
• Rohrbach S, Aurich AC, Li L, Niemann B. Age-associated loss in adiponectin-activation by caloric restriction: lack of compensation by enhanced inducibility of adiponectin paralogs CTRP2 and CTRP7. Mol Cell Endocrinol. 2007 Oct 15;277 (1-2):26-34
• Stejskal D, Karpisek M, Hanulova Z, Svestak M. Chemerin is an independent marker of the metabolic syndrome in a Caucasian population--a pilot study. Biomed Pap Med Fac Univ Palack. 2008 Dec;152 (2):217-21
• Tolman JR, Lephart ED, Setchell KD, Eggett DL, Christensen MJ. Timing of supplementation of selenium and isoflavones determines prostate cancer risk factor reduction in rats. Nutr Metab (Lond). 2008;5:31

References to summary

• Cherhab FF, Mounzih K, Lu R, Lim ME: Early onset of reproductive function in normal mice treated with leptin. Science 275, 88 (1997).
• Clement K, Vaisse C, Lahlou N, et al: A mutation in the human leptin receptor gene causes obesity and pituitary dysfunction. Nature 329, 398 (1998).
• Considine R.V., Sinha M.K., Heiman M.L., Kriaciunas A., Stephens T.W., Nyce M.R., Ohannesian J.P., Marco C.C., McKee L.J., Bauer T.L. and Caro J.F.: Serum immunoreactive leptin concentrations in normal-weight and obese humans. N. Engl. J. Med. 334, 292–295 (1996)
• Friedman JM, Halaas JL: Leptin and regulation of body weight inmammals. Nature 395, 763 (1998).
• Halaas J.L., Gajiwala K.S., Maffei M., Cohen S.L., Chait B.T., Rabinowitz D., Lallone R.L., Burley S.K. and Friedman J.M.: Weight-reducing effects of the plasma protein encoded by the obese gene. Science 269, 543–546 (1995)
• Montague CT, Faroozi IS, Witehead JP, ect: Congenital leptin deficiency deficiency is associated with serve early-onset obesity in humans. Nature 387, 903 (1997)
• Pelleymounter M.A., Cullen M.J., Baker M.B., Hecht R., Winters D., Boone T. and Collins F.: Effects of the obese gene product on body weight regulation in ob/ob mice. Science 269, 540–543 (1995)
• Zhang Y., Proenca R., Maffei M., Barone M., Leopold L., Friedman J.M.: Positional cloning of the mouse obese gene and its human homologue. Nature 372, 425–432 (1994)

By research topic:

• Animal studies
• Energy metabolism and body weight regulation
• Reproduction

By molecule:

• Leptin

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