Matrix Metalloproteinase-8 (MMP-8) Human ELISA

Summary

Matrix metalloproteinase 8 (MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells.1 The human neutrophil collagenase (HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein.2 Neu­trophil collagenase has been found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation.3 The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.

Research topic

Others

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Body fluids, Cell culture and/or animal studies, Plasma, Serum, Tissue lysates

Storage/Shipping

Store at 4°C for frequent use, at –20°C for infrequent use. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Calibration Curve

Calibration range

156 pg/ml-10 000 pg/ml

Limit of detection

< 10 pg/ml

References to summary

• Berliner N, Van Wart H, Mookhtiar K, Devarajan P. Structure and expression of the cDNA encoding human neutrophil collagenase. Blood. 1991 Jun 15;77 (12):2731-8
• Stevens RM, Spinella DG, Thompson JP, Goldberg GI, Pourmotabbed TF, Hasty KA, Mainardi CL. Human neutrophil collagenase. A distinct gene product with homology to other matrix metalloproteinases. J Biol Chem. 1990 Jul 15;265 (20):11421-4
• Strauss JF 3rd, Romero R, Shriver MD, Halder I, Tromp G, Kuivaniemi H, Ferrand PE, Sammel MD, Macones G, Parry S, Wang H. Functionally significant SNP MMP8 promoter haplotypes and preterm premature rupture of membranes (PPROM). Hum Mol Genet. 2004 Nov 1;13 (21):2659-69

By research topic:

• Others

By molecule:

• Matrix Metalloproteinase-8 (MMP-8)

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