MMP-9 Human ELISA

Notice

Restriction of a minimal quantity of 5 kits per order may apply.

Research topic

Others

Storage/Shipping

Store kit reagents between 2 – 8°C.

Summary

MMP-9 belongs to a family of enzymes with major actions in the remodelling of extracellular matrix components. It is the most complex family member in terms of domain structure and regulation of its activity. MMP-9 plays an important role in tumor invasion, angiogenesis and metastasis.

Assay format

Sandwich ELISA, Biotin-labelled antibody

Sample requirements

10 µl (1:25 prediluted)

Applications

Cell culture supernatant, Plasma-Citrate, Plasma-Heparin, Serum

Calibration Curve

Calibration range

0.23 – 15 ng/ml

Limit of detection

0.05 ng/ml

Limit of quantification

12.5 ng/ml

Intra-assay

CV = 7.3%

Inter-assay

CV = 10.2%

Spiking Recovery

103.1%

Dilution Linearity

107.4%

References to summary

• Dwek RA, Rudd PM, Nelissen I, Dubois B, Van den Steen PE, Opdenakker G. Biochemistry and molecular biology of gelatinase B or matrix metalloproteinase-9 (MMP-9). Crit Rev Biochem Mol Biol. 2002 Dec;37 (6):375-536
• Mobashery S, Meroueh SO, Chvyrkova I, Toth M, Fridman R. Cell surface association of matrix metalloproteinase-9 (gelatinase B). Cancer Metastasis Rev. 2003 Jun-Sep;22 (2-3):153-66
• Sellebjerg F, Sorensen TL. Chemokines and matrix metalloproteinase-9 in leukocyte recruitment to the central nervous system. Brain Res Bull. 2003 Aug 15;61 (3):347-55
• Nelissen I, Opdenakker G, Van Damme J. Functional roles and therapeutic targeting of gelatinase B and chemokines in multiple sclerosis. Lancet Neurol. 2003 Dec;2 (12):747-56
• Masure S, Van Coillie E, Nelissen I, Dubois B, Van den Steen PE, Opdenakker G, Proost P, Van Damme J. Gelatinase B functions as regulator and effector in leukocyte biology. J Leukoc Biol. 2001 Jun;69 (6):851-9
• Turpeenniemi-Hujanen T. Gelatinases (MMP-2 and -9) and their natural inhibitors as prognostic indicators in solid cancers. Biochimie. 2005 Mar-Apr;87 (3-4):287-97
• Lagente V, Boichot E, Belleguic C, Corbel M. Involvement of gelatinases (MMP-2 and MMP-9) in the development of airway inflammation and pulmonary fibrosis. Cell Biol Toxicol. 2002;18 (1):51-61
• Smits JF, Cleutjens JP, Creemers EE, Daemen MJ. Matrix metalloproteinase inhibition after myocardial infarction: a new approach to prevent heart failure?. Circ Res. 2001 Aug 3;89 (3):201-10
• Squire IB, Thompson MM. Matrix metalloproteinase-9 expression after myocardial infarction: physiological or pathological?. Cardiovasc Res. 2002 Jun;54 (3):495-8
• Senior RM, Atkinson JJ. Matrix metalloproteinase-9 in lung remodeling. Am J Respir Cell Mol Biol. 2003 Jan;28 (1):12-24
• Kanayama H. Matrix metalloproteinases and bladder cancer. J Med Invest. 2001 Feb;48 (1-2):31-43
• Van Muijen GN, Westphal JR, Hofmann UB, Ruiter DJ. Matrix metalloproteinases in human melanoma. J Invest Dermatol. 2000 Sep;115 (3):337-44
• Ohbayashi H. Matrix metalloproteinases in lung diseases. Curr Protein Pept Sci. 2002 Aug;3 (4):409-21
• Catt KJ, Shah BH. Matrix metalloproteinases in reproductive endocrinology. Trends Endocrinol Metab. 2004 Mar;15 (2):47-9
• van Kempen LC, Coussens LM. MMP9 potentiates pulmonary metastasis formation. Cancer Cell. 2002 Oct;2 (4):251-2
• Chakraborti T, Mandal A, Das S, Mandal M, Chakraborti S. Regulation of matrix metalloproteinases: an overview. Mol Cell Biochem. 2003 Nov;253 (1-2):269-85
• Vacirca J, Zucker S. Role of matrix metalloproteinases (MMPs) in colorectal cancer. Cancer Metastasis Rev. 2004 Jan-Jun;23 (1-2):101-17
• Jarjour NN, Kelly EA. Role of matrix metalloproteinases in asthma. Curr Opin Pulm Med. 2003 Jan;9 (1):28-33
• Lopez DM, Iragavarapu-Charyulu V, Owen JL. T cell-derived matrix metalloproteinase-9 in breast cancer: friend or foe?. Breast Dis. 2004;20:145-53
• de Bont ES, Kamps WA, Fraaije MW, Vellenga E, Klein G. The possible role of matrix metalloproteinase (MMP)-2 and MMP-9 in cancer, e.g. acute leukemia. Crit Rev Oncol Hematol. 2004 May;50 (2):87-100
• Yamashita J, Okada Y, Nakada M. The role of matrix metalloproteinases in glioma invasion. Front Biosci. 2003 Jan 1;8:e261-9
• Tuszynski G, John A. The role of matrix metalloproteinases in tumor angiogenesis and tumor metastasis. Pathol Oncol Res. 2001;7 (1):14-23