Monocyte Chemotactic Protein-1 Human ELISA

Research topic

Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation

Storage/Shipping

+4°C/ Wet ice

Summary

Monocyte chemotactic protein-1 (MCP-1) is a member of the C-C family of chemokines. Chemokines are mediators that share the capacity to induce directional migration of leukocytes. MCP-1 is a chemoattractant for many cell types such as monocytes, neutrophils, hepatic stellate cells, granulocytes and T lymphocytes. MCP-1 is believed to play a role in a variety of pathological conditions, including inflammatory and immune reactions, atherosclerosis and neoplasia. Monocyte Chemotactic Protein-1 (MCP-1) is also known as Monocyte Chemotactic Activating Factor (MCAF) or CCL2.

Assay format

Sandwich ELISA, Biotin-labelled antibody

Sample requirements

100 µl/well

Applications

Cell culture medium, Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum, Urine

Calibration Curve

Calibration range

16 to 1 000 pg/ml

Limit of detection

16 pg/ml

References to summary

• Soejima H et al.: Angiotensin-converting enzyme inhibition reduces monocyte chemoattractant protein-1 and tissue factor levels in patients with myocardial infarction.J Am Coll Cardiol 1999, 34: 982
• Oido-Mori M et al.: Porphyromonas gingivalis gingipain-R enhances interleukin-8 but decreases gamma interferon-inducible protein 10 production by human gingival fibroblasts in response to T-cell contact.Infect Immun 2001, 69: 4493
• Leeb S et al.: Regulation of migration of human colonic myofibroblasts­.Growth Factors 2002, 20: 81
• Petrek M et al.: CC and C chemokine expression in pulmonary sarcoidosis.Eur Respir J 2002, 20: 1206
• Pechkovsky D et al.: CCR2 and CXCR3 agonistic chemokines are differently expressed and regulated in human alveolar epithelial cells type II.Respir Res 2005, 6: 75