Monocyte Chemotactic Protein-1 Human ELISA, Sensitive

Summary

Monocyte chemotactic protein-1 (MCP-1) is a member of the C-C family of chemokines. Chemokines are mediators that share the capacity to induce directional migration of leukocytes. MCP-1 is a chemoattractant for many cell types such as monocytes, neutrophils, hepatic stellate cells, granulocytes and T lymphocytes. MCP-1 is believed to play a role in a variety of pathological conditions, including inflammatory and immune reactions, atherosclerosis and neoplasia. Monocyte Chemotactic Protein-1 (MCP-1) is also known as Monocyte Chemotactic Activating Factor (MCAF) or CCL2.

Research topic

Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Amniotic fluid, Cell culture supernatant, Plasma, Serum

Sample requirements

20 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

15.6 – 1000 pg/ml

Limit of detection

2.3 pg/ml

Intra-assay

CV = 4.7%

Inter-assay

CV = 8.7%

Spiking Recovery

92%

Dilution Linearity

105 %

By research topic:

• Cytokines and chemokines and related molecules
• Energy metabolism and body weight regulation

By molecule:

• Monocyte chemotactic protein 1