Monocyte Chemotactic Protein-1 Mouse ELISA

Summary

Monocyte chemotactic protein-1 (MCP-1) is a member of the C-C family of chemokines. Chemokines are mediators that share the capacity to induce directional migration of leukocytes. MCP-1 is a chemoattractant for many cell types such as monocytes, neutrophils, hepatic stellate cells, granulocytes and T lymphocytes. MCP-1 is believed to play a role in a variety of pathological conditions, including inflammatory and immune reactions, atherosclerosis and neoplasia. Monocyte Chemotactic Protein-1 (MCP-1) is also known as Monocyte Chemotactic Activating Factor (MCAF) or CCL2.

Research topic

Animal studies, Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Serum

Sample requirements

50 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

15.6 – 1000 pg/ml

Limit of detection

2.2 pg/ml

Intra-assay

CV = 6.2%

Inter-assay

CV = 5.6%

Spiking Recovery

98%

Dilution Linearity

97%

By research topic:

• Animal studies
• Cytokines and chemokines and related molecules
• Energy metabolism and body weight regulation

By molecule:

• Monocyte Chemotactic Protein-1