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Products\Immunoassays\NO Synthase I Human/Rat ELISA:
NO Synthase I Human/Rat ELISA
| Cat. No.: | RSCYK100R | Regulatory status: RUO | |
|---|---|---|---|
| Size: | 96 wells (1 kit) | | | |
| Files: | Datasheet PDF (RUO) | ||
| Legend: |  new product discount |
 temporarily out of stock order in advance |
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Research topic
Animal studies, Blood pressure regulation and NO metabolism
Storage/Shipping
Store all of the components at 2–8°C.
Summary
NO Synthase is an enzyme in the body that contributes to transmission from one neuron to another, to the immune system and to dilating blood vessels. It does so by synthesis of nitric oxide from the terminal nitrogen atom of L-arginine in the presence of NADPH and dioxygen. NOS is the only known enzyme that binds flavin adenine dinucleotide, flavin mononucleotide, heme, tetrahydrobiopterin and calmodulin.
Assay format
Competitive ELISA, Immobilized antibody
Sample requirements
50 µl/well
Applications
Tissue extract
Calibration Curve
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Calibration range
0.412 to 100 ng/ml
Intra-assay
4.0 – 5.3 %
Inter-assay
4.7 – 8.0 %
References to summary
- Nakane M et al.: Cloned human brain nitric oxide synthase is highly expressed in skeletal muscle.FEBS 316, 175–180 (1993).
- Imai T et al.: Expression of brain nitric oxide synthase mRNA in various tissues and cultured cells of rat.Biomed Res 13, 371–374 (1992).
- Schmidt HHHW et al.: Biochemistry and regulation of nitric oxide synthase.Taniguchi Symposium on Brain Sciences No.17, 3–18 (1994).
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