NO Synthase I Human/Rat ELISA

Summary

NO Synthase is an enzyme in the body that contributes to transmission from one neuron to another, to the immune system and to dilating blood vessels. It does so by synthesis of nitric oxide from the terminal nitrogen atom of L-arginine in the presence of NADPH and dioxygen. NOS is the only known enzyme that binds flavin adenine dinucleotide, flavin mononucleotide, heme, tetrahydrobiopterin and calmodulin.

Research topic

Animal studies, Blood pressure regulation and NO metabolism

Assay format

Competitive ELISA, Immobilized antibody

Applications

Tissue extract

Sample requirements

50 µl/well

Storage/Shipping

Store all of the components at 2–8°C.

Calibration Curve

Calibration range

0.412 to 100 ng/ml

Intra-assay

4.0 – 5.3 %

Inter-assay

4.7 – 8.0 %

References to summary

• Nakane M et al.: Cloned human brain nitric oxide synthase is highly expressed in skeletal muscle.FEBS 316, 175–180 (1993).
• Imai T et al.: Expression of brain nitric oxide synthase mRNA in various tissues and cultured cells of rat.Biomed Res 13, 371–374 (1992).
• Schmidt HHHW et al.: Biochemistry and regulation of nitric oxide synthase.Taniguchi Symposium on Brain Sciences No.17, 3–18 (1994).

By research topic:

• Animal studies
• Blood pressure regulation and NO metabolism

By molecule:

• NO Synthase