Obestatin Human ELISA

Research topic

Energy metabolism and body weight regulation

Assay format

Competitive ELISA, Immobilized antibody

Applications

Plasma, Serum

Sample requirements

20 μl

Storage/Shipping

Store all the components at 2 to 8°C.

Calibration Curve

Calibration range

0.412–100 ng/ml

Intra-assay

CV = 3.5–9.9%

Inter-assay

CV = 5.6–9.0%

Spiking Recovery

Human serum: 101.5–113.2% Human plasma: 106.1–118.9%

Dilution Linearity

Linear dilution characteristics were shown with human serum and human plasma at least up to 8 folds and 4 folds respectively.

References to summary

• Nagaraj S, Peddha MS, Manjappara UV. Fragments of obestatin as modulators of feed intake, circulating lipids, and stored fat. Biochem Biophys Res Commun. 2008 Feb 15;366 (3):731-7
• Ren AJ, Guo ZF, Wang YK, Wang LG, Wang WZ, Lin L, Zheng X, Yuan WJ. Inhibitory effect of obestatin on glucose-induced insulin secretion in rats. Biochem Biophys Res Commun. 2008 May 9;369 (3):969-72
• Scrima M, Campiglia P, Esposito C, Gomez-Monterrey I, Novellino E, D'Ursi AM. Obestatin conformational features: a strategy to unveil obestatin's biological role?. Biochem Biophys Res Commun. 2007 Nov 23;363 (3):500-5

By research topic:

• Energy metabolism and body weight regulation

By molecule:

• Obestatin