Obestatin Mouse/Rat ELISA

Research topic

Animal studies, Energy metabolism and body weight regulation

Assay format

Competitive ELISA, Immobilized antibody

Applications

Serum

Sample requirements

25 μl

Storage/Shipping

Store all the components at 2 to 8°C.

Calibration Curve

Calibration range

0.082–20 ng/ml

Intra-assay

mouse serum: CV = 3.7–6.9% rat serum: CV= 3.4–6.7%

Inter-assay

mouse serum: CV = 4.5–8.4% rat serum: CV= 8.1–10.8%

Spiking Recovery

Mouse serum: 102.7–108.9% Rat serum: 85.7–95.7%

Dilution Linearity

Satisfactory dilution characteristics were shown with mouse and rat serum.

References to summary

• Green BD, Irwin N, Flatt PR. Direct and indirect effects of obestatin peptides on food intake and the regulation of glucose homeostasis and insulin secretion in mice. Peptides. 2007 May;28 (5):981-7
• Dun SL, Brailoiu GC, Brailoiu E, Yang J, Chang JK, Dun NJ. Distribution and biological activity of obestatin in the rat. J Endocrinol. 2006 Nov;191 (2):481-9
• Samson WK, White MM, Price C, Ferguson AV. Obestatin acts in brain to inhibit thirst. Am J Physiol Regul Integr Comp. 2007 Jan;292 (1):R637-43
• Zhang JV, Ren PG, Avsian-Kretchmer O, Luo CW, Rauch R, Klein C, Hsueh AJ. Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin's effects on food intake. Science. 2005 Nov 11;310 (5750):996-9

By research topic:

• Animal studies
• Energy metabolism and body weight regulation

By molecule:

• Obestatin