Omentin-1 Human ELISA

Summary

Omentin (intelectin-1, intestinal lactoferin receptor, endothelian lectin HL-1, galactofurano­sebinding lectin) is newly identified secretory protein that is highly and selectively expressed in visceral adipose tissue relative to subcutaneous adipose tissue (adipokine). The mature omentin is secretory glycoprotein consisting of 295 amino acids and 1-linked oligosacharides, and its basic structural unit is a 120-kDa homotrimer in which 40-kDa polipeptides are bridged by disulfide bonds. Omentin has been identified in other tissue at lower expression levels such are Paneth cells, endothelial cells, and visceral adipose stromal-vascular cells. A homolog of omentin has been identified that shares 83% amino acid identity with omentin and was referred to as omentin 2. The two omentin genes, omentin 1 and omentin 2, are localized adjacent to each other in chromosomal region, which has been previously linked to type 2 diabetes in several populations. To determine the impact of obesity-dependent insulin resistance on the regulation of two omentin isoforms, gene expression and plasma levels were measured in lean, overweight, and obese subjects. Omentin-1 was shown to be the major circulating isoform in human plasma. Lean subjects had significantly higher omentin-1 plasma levels than obese and overweight subjects. In addition, higher plasma omentin-1 levels were detected in women compared with men. Plasma omentin-1 levels were inversely correlated with BMI, waist circumference, leptin levels, and insulin resistance as measured by homeostasis model assessment and positively correlated with adiponectin and HDL levels. In summary, decreased omentin-1 levels are associated with increasing obesity and insulin resistance. An independent experiment reported that the addition of recombinant omentin-1 in vitro did not affect basal glucose uptake but did enhance insulin-stimulated glucose uptake in both subcutaneous and omental human adipocytes. Omentin-1 increased Akt phosphorylation in the absence and presence of insulin and may regulate insulin action. A recent study of women with the polycystic ovary syndrome (PCOS) found significantly reduced omentin-1 mRNA expression and protein levels in adipose tissue in overweight PCOS women. In addition, significantly lower plasma omentin-1 levels were detected in these women.

Features

• It is intended for research use only.
• The total assay time is less than 3.5 hours.
• The kit measures omentin-1 in serum and plasma (EDTA, citrate, heparin).
• Assay format is 96 wells.
• Quality Controls are human serum based. No animal sera are used.
• Standard is recombinant protein based.
• Components of the kit are provided ready to use, concentrated or lyophilized.

Research topic

Diabetology - Other Relevant Products, Energy metabolism and body weight regulation, Reproduction

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum

Sample requirements

6 ul

Calibration Curve

Calibration range

2 to 64 ng/ml

Limit of detection

0.5 ng/ml

Intra-assay (Within-Run, n=8)

CV = 3.7%

Inter-assay (Run-to-Run, n=8)

CV = 4.6%

Spiking Recovery

94.8%

Dilution Linearity

104.1%

Cross-Reactivity

References to this product

• Hamdy NM, El-Derany MO, El-Mesallamy HO. Serum omentin-1 and chemerin levels are interrelated in patients with Type 2 diabetes mellitus with or without ischaemic heart disease. Diabet Med. 2011 Oct;28 (10):1194-200
• Murohara T, Kihara S, Ikeda N, Ohashi K, Kataoka Y, Takeshita K, Takahashi R, Kikuchi R, Ouchi N, Shibata R. Circulating omentin is associated with coronary artery disease in men. Atherosclerosis. 2011 Dec;219 (2):811-4
• Sung KC, Lee WY, Woo HY, Kim KH, Rhee EJ, Choi JH. Plasma omentin-1 levels are reduced in non-obese women with normal glucose tolerance and polycystic ovary syndrome. Eur J Endocrinol. 2011 Nov;165 (5):789-96

References to summary

• Adya R, Tan BK, O'Hare P, Farhatullah S, Lehnert H, Lewandowski KC, Randeva HS. Omentin-1, a novel adipokine, is decreased in overweight insulin-resistant women with polycystic ovary syndrome: ex vivo and in vivo regulation of omentin-1 by insulin and glucose. Diabetes. 2008 Apr;57 (4):801-8
• Avsar E, Kalayci C, Imeryuz N, Celikel CA, Ozdogan O, Eren F, Alahdab YO, Kurt R, Yonal O, Yilmaz Y. Serum levels of omentin, chemerin and adipsin in patients with biopsy-proven nonalcoholic fatty liver disease. Scand J Gastroenterol. 2011 Jan;46 (1):91-7
• Buechler C, Schaffler A, Weigert J, Neumeier M, Wurm S. Plasma levels of leptin, omentin, collagenous repeat-containing sequence of 26-kDa protein (CORS-26) and adiponectin before and after oral glucose uptake in slim adults. Cardiovasc Diabetol. 2007;6:7
• Fernandez-Real JM, Fruhbeck G, Ricart W, Gomez-Ambrosi J, Ortega F, Catalan V, Moreno-Navarrete JM. Circulating omentin concentration increases after weight loss. Nutr Metab (Lond). 2010;7:27
• McLenithan JC, Pollin TI, Shuldiner AR, Gong DW, Fried SK, Kligman M, Schwartz A, Patil S, Ndubuizu K, Yu DZ, Pray J, Glynn NM, Lee MJ, Yang RZ, de Souza Batista CM. Omentin plasma levels and gene expression are decreased in obesity. Diabetes. 2007 Jun;56 (6):1655-61
• Neumeier M, Herfarth H, Scholmerich J, Buchler C, Schaffler A, Furst A. Genomic structure of human omentin, a new adipocytokine expressed in omental adipose tissue. Biochim Biophys Acta. 2005 Dec 30;1732 (1-3):96-102
• Pan HY, Guo L, Li Q. Changes of serum omentin-1 levels in normal subjects and in patients with impaired glucose regulation and with newly diagnosed and untreated type 2 diabetes. Diabetes Res Clin Pract. 2010 Apr;88 (1):29-33
• Pray J, Yang RZ, Gong DW, McLenithan JC, Lee MJ, Fried SK, Shuldiner AR, Hu H, Hansen BC, Wu HB. Identification of omentin as a novel depot-specific adipokine in human adipose tissue: possible role in modulating insulin action. Am J Physiol Endocrinol Metab. 2006 Jun;290 (6):E1253-61
• Randeva HS, Lehnert H, Lewandowski KC, O'Hare P, Farhatullah S, Adya R, Tan BK. Omentin-1, a novel adipokine, is decreased in overweight insulin-resistant women with polycystic ovary syndrome: ex vivo and in vivo regulation of omentin-1 by insulin and glucose. Diabetes. 2008 Apr;57 (4):801-8
• Yamawaki H, Tsubaki N, Mukohda M, Okada M, Hara Y. Omentin, a novel adipokine, induces vasodilation in rat isolated blood vessels. Biochem Biophys Res Commun. 2010 Mar 19;393 (4):668-72

By research topic:

• Diabetology - Other Relevant Products
• Energy metabolism and body weight regulation
• Reproduction

By molecule:

• Omentin-1

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