Type
Sandwich ELISA, HRP-labelled antibody
Description
The RIS002R Human Osteocalcin ELISA Immunoenzymetric assay for the measurement of intact human osteocalcin (OST) in serum. It is intended for research use only.
Applications
Serum
Sample Requirements
25 µl/well
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
1.56–75 ng/ml
Limit of Detection
0.08 ng/ml
Intra-assay (Within-Run)
CV < 6%
Inter-assay (Run-to-Run)
CV < 6%
Features
- RUO
- calibration range 1.56-75 ng/ml
- limit of detection 0.08ng/ml
- quality controls
Research topic
Bone and cartilage metabolism
Summary
Biological activities
Osteocalcin or bone Gla protein (B.G.P) is the major non-collagen protein of the bone matrix. It has a molecular weight of 5800 Da and contains 49 amino-acids, including 3 residues of gamma carboxyl glutamic acid. Osteocalcin is synthesized in the bone by the osteoblasts. After production, it is partly incorporated in the bone matrix and the rest is found in the blood circulation. The exact physiological function of osteocalcin is still unclear. A large number of studies show that the circulating levels of osteocalcin reflect the rate of bone formation.
Clinical application
The determination of the blood levels of osteocalcin is valuable for :
− The identification of women at risk of developing osteoporosis
− Monitoring bone metabolism during the perimenopause and postmenopause
− Monitoring bone metabolism during hormone replacement therapy and treatment of premenopausal women with LH-RH agonists
− Monitoring bone metabolism in patients with growth hormone deficiency, hypothyroidism, hyperthyroidism, chronic renal failure.
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
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