p53 Human ELISA

Summary

p53 is the most commonly mutated gene in human cancer. It is highly conserved and expressed in most normal tissues. p53 is considered a stress response gene, and acts to induce cell cycle arrest or apoptosis in response to DNA damage, thereby maintaining genetic stability in the organism. It is a tumor suppressor gene which induces apoptosis.

Research topic

Oncology

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Serum

Sample requirements

50 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

0.8 – 50 U/ml

Limit of detection

0.33 U/ml

Intra-assay

CV = 5.5%

Inter-assay

CV = 8.9%

Spiking Recovery

95%

Dilution Linearity

102 %

By research topic:

• Oncology

By molecule:

• p53

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