PAI-1 Human ELISA

Summary

PAI-1 is the primary inhibitor of plasminogen activators in plasma. PAI-1 is a single-chain glycoprotein with a molecular weight of 47 kDa. It is synthesized in the liver and by endothelial cells, and its synthesis is regulated by several physiologic mediators including endotoxin, interleukin-1, fibroblast growth factor-2, and lipids. PAI-1 is an important inhibitor of the fibrinolytic system, therefore, elevated levels could suppress fibrinolysis and result in an increased risk of thrombosis.

Research topic

Others

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Plasma, Serum

Sample requirements

50 µl (1:50 prediluted)

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

78 – 5000 pg/ml

Limit of detection

29 pg/ml

Intra-assay

CV = 4.7%

Inter-assay

CV = 5.0%

Spiking Recovery

58.7%

Dilution Linearity

98.7%

References to summary

• Adachi MM, Gottfried EL. Prothrombin time and activated partial thromboplastin time can be performed on the first tube. Am J Clin Pathol. 1997 Jun;107 (6):681-3
• Gulba D, Wojta J, Christ G, Huber K. Plasminogen activator inhibitor type-1 in cardiovascular disease. Status report 2001. Thromb Res. 2001 Sep 30;103 Suppl 1:S7-19
• Huber K. Plasminogen activator inhibitor type-1 (part one): basic mechanisms, regulation, and role for thromboembolic disease. J Thromb Thrombolysis. 2001 May;11 (3):183-93
• Huber K. Plasminogen activator inhibitor type-1 (part two): role for failure of thrombolytic therapy. PAI-1 resistance as a potential benefit for new fibrinolytic agents. J Thromb Thrombolysis. 2001 May;11 (3):195-202
• Marlar RA, Kressin DC, Adcock DM. Effect of 3.2% vs 3.8% sodium citrate concentration on routine coagulation testing. Am J Clin Pathol. 1997 Jan;107 (1):105-10
• National Committee for Clinical Laboratory Standardization. Collection, Transport, and Processing of Blood Specimens for Coagulation Testing and General Performance of Coagulation Assays; Approved Guideline. Third Edition, Villanova: NCCL. 1999;H21-A3:11(23)
• Ng VL, Leslie S, Gottfried EL, Reneke J, Etzell J. Prolonged prothrombin time and activated partial thromboplastin time due to underfilled specimen tubes with 109 mmol/L (3.2%) citrate anticoagulant. Am J Clin Pathol. 1998 Jun;109 (6):754-7
• Ray H, McGlasson DL, More L, Norris WL, Best HA, Doe RH. Drawing specimens for coagulation testing: is a second tube necessary?. Clin Lab Sci. 1999 May-Jun;12 (3):137-9

By research topic:

• Others

By molecule:

• PAI-1

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