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Human PAI-1 ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Plasminogen activator inhibitor 1, Endothelial plasminogen activator inhibitor, Serpine 1, PAI, PLANH-1
  • Species:Human
Cat. No. Size Price


RAF083R 96 wells (1 kit) $721,83
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Description

The Human PAI-1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human PAI-1. The Human PAI-1 ELISA is for research use only. Not for use in diagnostic procedures.

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate, Cell culture supernatant

Sample Requirements

50 µl (1:50 prediluted)

Shipping

On blue ice packs. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

78–5000 pg/ml

Limit of Detection

29 pg/ml

Intra-assay (Within-Run)

CV = 4.7%

Inter-assay (Run-to-Run)

CV = 5.0%

Spiking Recovery

serum 72.2%, plasma 58.7%

Dilution Linearity

98,70%

Specificity

The assay detects both natural and recombinant human PAI-1. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into serum. No cross-reactivity was detected.

Summary

Features

  • RUO
  • calibration range 78-5000 pg/ml
  • limit of detection 29 pg/ml
  • intra-assay CV = 4.7%
  • inter-assay CV = 5.0%

Research topic

Energy metabolism and body weight regulation, Others

Summary

Plasminogen activator inhibitor-1 (PAI-1) is the primary inhibitor of plasminogen activators in plasma, rapidly inactivating both tissue plasminogen activator (t-PA) and urokinase-type plasminogen activator (u-PA). PAI-1 is a single-chain glycoprotein with a molecular weight of 47 kilodaltons. During fibrinolysis, tissue plasminogen activator (tPA) converts the inactive protein plasminogen into plasmin which plays a critical role in fibrinolysis by degrading fibrin and providing localized protease activity in a number of physiological functions. PAI-1 is synthesized in the liver and by endothelial cells, and its synthesis is regulated by several physiologic mediators, including endotoxin, interleukin-1, fibroblast growth factor-2, and lipids. Plasminogen activator inhibitor-1 is an important inhibitor of the fibrinolytic system, so elevated levels could suppress fibrinolysis and result in an increased risk of thrombosis. Increased PAI-1 levels have been shown to be associated with a number of atherosclerotic risk factors, PAI-1 has been shown to act as a prothrombic factor in both arterial and venous thromboembolic disorders.

Summary References (8)

References to PAI-1

  • Adcock DM, Kressin DC, Marlar RA. Effect of 3.2% vs 3.8% sodium citrate concentration on routine coagulation testing. Am J Clin Pathol. 1997 Jan;107 (1):105-10
  • Gottfried EL, Adachi MM. Prothrombin time and activated partial thromboplastin time can be performed on the first tube. Am J Clin Pathol. 1997 Jun;107 (6):681-3
  • Huber K. Plasminogen activator inhibitor type-1 (part one): basic mechanisms, regulation, and role for thromboembolic disease. J Thromb Thrombolysis. 2001 May;11 (3):183-93
  • Huber K. Plasminogen activator inhibitor type-1 (part two): role for failure of thrombolytic therapy. PAI-1 resistance as a potential benefit for new fibrinolytic agents. J Thromb Thrombolysis. 2001 May;11 (3):195-202
  • Huber K, Christ G, Wojta J, Gulba D. Plasminogen activator inhibitor type-1 in cardiovascular disease. Status report 2001. Thromb Res. 2001 Sep 30;103 Suppl 1:S7-19
  • McGlasson DL, More L, Best HA, Norris WL, Doe RH, Ray H. Drawing specimens for coagulation testing: is a second tube necessary?. Clin Lab Sci. 1999 May-Jun;12 (3):137-9
  • National Committee for Clinical Laboratory Standardization. Collection, Transport, and Processing of Blood Specimens for Coagulation Testing and General Performance of Coagulation Assays; Approved Guideline. Third Edition, Villanova: NCCL. 1999;H21-A3:11(23)
  • Reneke J, Etzell J, Leslie S, Ng VL, Gottfried EL. Prolonged prothrombin time and activated partial thromboplastin time due to underfilled specimen tubes with 109 mmol/L (3.2%) citrate anticoagulant. Am J Clin Pathol. 1998 Jun;109 (6):754-7
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