Perforin Human ELISA

Summary

Perforin has structural and functional similarities to complement component 9 (C9). Like C9, Perforin creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood.

Research topic

Apoptosis

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Buffer solution, Cell culture supernatant

Sample requirements

100 µl/well

Storage/Shipping

2–8 °C/Wet ice

Calibration Curve

Calibration range

62.5 to 2000 pg/ml

Limit of detection

< 40 pg/ml

References to this product

• Cousins RJ, McClellan S, Liuzzi JP, Aydemir TB. Zinc transporter ZIP8 (SLC39A8) and zinc influence IFN-gamma expression in activated human T cells. J Leukoc Biol. 2009 Aug;86 (2):337-48
• Klotz SA, Schmidt JO, Pinnas JL, Stevens L, Logan JL, Dorn PL, Klotz JH. "Kissing bugs": potential disease vectors and cause of anaphylaxis. Clin Infect Dis. 2010 Jun 15;50 (12):1629-34
• Mingari MC, Moretta L, Azzarone B, Moretti S, Cottalasso F, Vitale C, Chiossone L. Molecular analysis of the methylprednisolone-mediated inhibition of NK-cell function: evidence for different susceptibility of IL-2- versus IL-15-activated NK cells. Blood. 2007 May 1;109 (9):3767-75
• Norgauer J, Maghazachi AA, Kamradt T, Barz D, Idzko M, Ziemer M, Berod L, Lehmann K, Truta-Feles K, Lagadari M. Lysophosphatidic acid inhibits the cytotoxic activity of NK cells: involvement of Gs protein-mediated signaling. Int Immunol. 2009 Jun;21 (6):667-77
• Stingl G, Kopp T, Strohal R, Tauber M, Bangert C, Stary G. Tumoricidal activity of TLR7/8-activated inflammatory dendritic cells. J Exp Med. 2007 Jun 11;204 (6):1441-51

By research topic:

• Apoptosis

By molecule:

• Perforin