Phosphorylated Neurofilament H Human ELISA

Features

• It is intended for research use only.
• The total assay time is less than 4 hours.
• The kit measures pNF-H in serum, plasma, cerebrospinal fluid (CSF) and tissue samples
• Assay format is 96 wells.
• Standard and Quality Controls are human brain extract based. No animal sera are used.
• Components of the kit are provided ready to use, concentrated or lyophilized.

Research topic

Neural tissue damage markers

Find out more on biovendor.com

• Hyperphosphorylated neurofilament NF-H as a predictor of mortality after brain injury in children in BioVendor News

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Cerebrospinal fluid, Plasma, Serum, Tissue extract

Storage/Shipping

Store the complete kit at 2 8°C.

Calibration Curve

Calibration range

62.5 – 4000 pg/ml

Limit of detection

23.5 pg/ml

Intra-assay (Within-Run, n=8)

CV = 4.5%

Spiking Recovery

CSF sample: 98.8% Serum sample: 95.8%

Dilution Linearity

CSF sample: 106.1% Serum sample: 105.5%

References to this product

• Zurek J, Bartlova L, Fedora M. Hyperphosphorylated neurofilament NF-H as a predictor of mortality after brain injury in children. Brain Inj. 2011;25 (2):221-6
• Zurek J, Fedora M. The usefulness of S100B, NSE, GFAP, NF-H, secretagogin and Hsp70 as a predictive biomarker of outcome in children with traumatic brain injury. Acta Neurochir (Wien). 2011 Oct 7;

References to summary

• Anderson KJ, Scheff SW, Miller KM, Roberts KN, Gilmer LK, Yang C, Shaw G. The phosphorylated axonal form of the neurofilament subunit NF-H (pNF-H) as a blood biomarker of traumatic brain injury. J Neurotrauma. 2008 Sep;25 (9):1079-85
• Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Iqbal K, Grundke-Iqbal I, Wang JZ. Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neurosci Lett. 2002 Mar 8;320 (3):156-60
• Lewis SB, Wolper RA, Miralia L, Yang C, Shaw G. Detection of phosphorylated NF-H in the cerebrospinal fluid and blood of aneurysmal subarachnoid hemorrhage patients. J Cereb Blood Flow Metab. 2008 Jun;28 (6):1261-71
• Petzold A. Neurofilament phosphoforms: surrogate markers for axonal injury, degeneration and loss. J Neurol Sci. 2005 Jun 15;233 (1-2):183-98
• Petzold A, Rejdak K, Plant GT. Axonal degeneration and inflammation in acute optic neuritis. J Neurol Neurosurg Psychiatry. 2004 Aug;75 (8):1178-80
• Petzold A, Shaw G. Comparison of two ELISA methods for measuring levels of the phosphorylated neurofilament heavy chain. J Immunol Methods. 2007 Jan 30;319 (1-2):34-40
• Shaw G, Yang C, Ellis R, Anderson K, Parker Mickle J, Scheff S, Pike B, Anderson DK, Howland DR. Hyperphosphorylated neurofilament NF-H is a serum biomarker of axonal injury. Biochem Biophys Res Commun. 2005 Nov 4;336 (4):1268-77

By research topic:

• Neural tissue damage markers

By molecule:

• Phosphorylated Neurofilament H

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