Phosphorylated Neurofilament H Human ELISA

Features

• It is intended for research use only.
• The total assay time is less than 4 hours.
• The kit measures pNF-H in serum, plasma, cerebrospinal fluid (CSF) and tissue samples
• Assay format is 96 wells.
• Standard and Quality Controls are human brain extract based. No animal sera are used.
• Components of the kit are provided ready to use, concentrated or lyophilized.

Research topic

Neural tissue damage markers

Find out more on biovendor.com

• Hyperphosphorylated neurofilament NF-H as a predictor of mortality after brain injury in children in BioVendor News

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Cerebrospinal fluid, Plasma, Serum, Tissue extract

Storage/Shipping

Store the complete kit at 2 8°C.

Calibration Curve

Calibration range

62.5 – 4000 pg/ml

Limit of detection

23.5 pg/ml

Intra-assay (Within-Run, n=8)

CV = 4.5%

Spiking Recovery

CSF sample: 98.8% Serum sample: 95.8%

Dilution Linearity

CSF sample: 106.1% Serum sample: 105.5%

References to this product

• Fedora M, Bartlova L, Zurek J. Hyperphosphorylated neurofilament NF-H as a predictor of mortality after brain injury in children. Brain Inj. 2011;25 (2):221-6
• Fedora M, Zurek J. The usefulness of S100B, NSE, GFAP, NF-H, secretagogin and Hsp70 as a predictive biomarker of outcome in children with traumatic brain injury. Acta Neurochir (Wien). 2011 Oct 7;

References to summary

• Howland DR, Anderson DK, Pike B, Scheff S, Parker Mickle J, Anderson K, Ellis R, Yang C, Shaw G. Hyperphosphorylated neurofilament NF-H is a serum biomarker of axonal injury. Biochem Biophys Res Commun. 2005 Nov 4;336 (4):1268-77
• Petzold A. Neurofilament phosphoforms: surrogate markers for axonal injury, degeneration and loss. J Neurol Sci. 2005 Jun 15;233 (1-2):183-98
• Plant GT, Rejdak K, Petzold A. Axonal degeneration and inflammation in acute optic neuritis. J Neurol Neurosurg Psychiatry. 2004 Aug;75 (8):1178-80
• Shaw G, Petzold A. Comparison of two ELISA methods for measuring levels of the phosphorylated neurofilament heavy chain. J Immunol Methods. 2007 Jan 30;319 (1-2):34-40
• Shaw G, Yang C, Gilmer LK, Roberts KN, Miller KM, Scheff SW, Anderson KJ. The phosphorylated axonal form of the neurofilament subunit NF-H (pNF-H) as a blood biomarker of traumatic brain injury. J Neurotrauma. 2008 Sep;25 (9):1079-85
• Shaw G, Yang C, Miralia L, Wolper RA, Lewis SB. Detection of phosphorylated NF-H in the cerebrospinal fluid and blood of aneurysmal subarachnoid hemorrhage patients. J Cereb Blood Flow Metab. 2008 Jun;28 (6):1261-71
• Wang JZ, Grundke-Iqbal I, Iqbal K, Khatoon S, Duan QH, Wang XC, He SS, Hu YY. Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neurosci Lett. 2002 Mar 8;320 (3):156-60

By research topic:

• Neural tissue damage markers

By molecule:

• Phosphorylated Neurofilament H

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