PMN Elastase Human ELISA

Summary

The human organism reacts with an inflammatory response to attacks of invading pathogens (micro-organisms and viruses) or damaged tissue (after accidents or surgery). Polymorphonuclear (PMN) granulocytes play an important role as primary defence cells in this inflammatory reaction. Different bloodstream mediators (cytokines, leukotrienes, complement factors, bacterial endotoxins, clotting and fibrinolysis factors) attract and stimulate these cells to phagocytize and destroy not naturally occurring agents. PMN granulocytes use proteinases to digest these agents and tissue debris. One of these proteinases is PMN elastase which is localised in the azurophilic granules of the polymorphonuclear granulocytes. During phagocytosis of foreign substances these enzymes are also partially excreted into the extracellular surrounding, where the aktivity of PMN elastase is regulated by inhibitors (esp. the α1-proteinase inhibitor, α1-PI). An overwhelming release of PMN elastase, however, can exceed the inhibitory potential of the α1-proteinase inhibitor. Thus, enzymatically active PMN elastase, together with simultaneously produced oxidants (O₂-radicals, H₂O₂, OH-radicals), can cause local tissue injury. Due to the bloodstream and lymphatic system, however, α1-PI is delivered subsequently and eventually able to form a complex with all excreted elastase. Therefore, the concentration of the PMN elastase/ α1-PI complex correlates with the released PMN elastase and can be used as a measure for the activity of granulocytes during an inflammatory response. Primarily, determinations of PMN elastase find its application in observation of the course of trauma, shock and sepsis. Further indications are the areas of hemodialysis, infections by obstetrics, joint diseases, effusions of sport injuries, intestinal affection, pancreatitis, cystic fibrosis and male adnex affections.

Features

• European Union: for in vitro diagnostic use. Rest of the world: for research use only!
• The total assay time is less than 3 hours.
• The kit measures PMN elastase in plasma (EDTA and citrate)
• Assay format is 96 wells.
• Components of the kit are provided ready to use, concentrated or lyophilized.

Research topic

Immune Response, Infection and Inflammation

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Plasma-Citrate, Plasma-EDTA

Sample requirements

10 µl/well

Storage/Shipping

Store the kit at 2 – 8°C. Storage conditions for aliquots of Standards, Quality Controls and samples are – 20°C. Shelf life of components is 30 days after opening or until the expiration date.

Calibration Curve

Calibration range

15.6 – 1000 ng/ml

Limit of detection

3 ng/ml

Intra-assay

4.8 %

Inter-assay (Run-to-Run, n=5)

CV = 5.6 %

Spiking Recovery

104 %

Dilution Linearity

97 %

Cross-Reactivity

References to this product

• Allan EK, Holyoake TL, Jorgensen HG. .
• Chimenti L, Morici G, Paterno A, Bonanno A, Vultaggio M, Bellia V, Bonsignore MR. Environmental conditions, air pollutants, and airway cells in runners: a longitudinal field study. J Sports Sci. 2009 Jul;27 (9):925-35
• Laing SJ, Jackson AR, Walters R, Lloyd-Jones E, Whitham M, Maassen N, Walsh NP. Human blood neutrophil responses to prolonged exercise with and without a thermal clamp. J Appl Physiol. 2008 Jan;104 (1):20-6
• Naskalski JW, Kapusta M, Fedak D, Dumnicka P, Kusnierz-Cabala B, Kuzniewski M, Sulowicz W. Effect of Hemodialysis on Acid Leukocyte-Type Ribonuclease, Alkaline Ribonuclease and Polymorphonuclear Elastase Serum Levels in Patients with End-Stage Renal Disease. Nephron Clin Pract. 2009 Jun 16;112 (4):c248-c254
• Park CJ, Clark SG, Lichtensteiger CA, Jamison RD, Johnson AJ. Accelerated wound closure of pressure ulcers in aged mice by chitosan scaffolds with and without bFGF. Acta Biomater. 2009 Jul;5 (6):1926-36

References to summary

• Jochum M., Machleidt, W., Neuhof, H., and Fritz, H. Proteinases. In: Schlag G, Redl H (eds), Pathophysiology of Shock, Sepsis, and Organ Failure. Springer-Verlag Berlin Heidelberg, 1993: 46–60.
• Jochum M., Machleidt, W., and Fritz, H. Proteolytic Enzyme Systems. In: Schlag G, Redl H (eds), Pathophysiology of Shock, Sepsis, and Organ Failure. Springer-Verlag Berlin Heidelberg, 1993: 531–548.
• Elastase In: Thomas, L. (Hrsg.) Labor und Diagnose Die Medizinische Verlagsgesellschaf­t, 4. Auflage, 1992: 795 – 801
• Elastase, Elastase-α1-Proteinase Inhibitor Complex In: Friedman, R.B., Young, D.S (Eds.) Effects of Disease on Clinical Laboratory Tests AACC Press, Washington, 3rd Edition, 1997: 3–161
• Reinhardt, A., Haidl, G., and Schill, W-B. Granulocyte elastase indicates silent male genital tract inflammation and appropriate antiinflammatory treatment. Andrologia 29, 1996: 187 – 192

By research topic:

• Immune Response, Infection and Inflammation

By molecule:

• PMN Elastase

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