Progesterone ELISA

Summary

Progesterone is a C-21 female sex steroid hormone with a variety of physiological effects. In the follicular phase of the menstrual cycle, progesterone is produced in low levels. It increases to the LH peak and then sharply rises 3 to 4 days later to higher levels. Progesterone measurements are used in documenting ovulation and in the management of difficulties during the first trimester of pregnancy. Levels of progesterone may be useful in the evaluation of sterility due to luteal phase defects, prediction of impending abortion, and the diagnosis of ectopic pregnancy.

Research topic

Steroid hormones

Assay format

Competitive ELISA, Immobilized antigen

Applications

Serum

Calibration Curve

Calibration range

0 – 60 ng/ml

Limit of detection

0.1 ng/ml

Intra-assay

CV = 10.4%

Inter-assay

CV = 11.4%

Spiking Recovery

98%

Dilution Linearity

93%

By research topic:

• Steroid hormones

By molecule:

• Progesterone